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C57BL/6JCya-Ago1em1flox/Cya
Common Name:
Ago1-flox
Product ID:
S-CKO-18237
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ago1-flox
Strain ID
CKOCMP-236511-Ago1-B6J-VB
Gene Name
Ago1
Product ID
S-CKO-18237
Gene Alias
Eif2c1
Background
C57BL/6JCya
NCBI ID
236511
Modification
Conditional knockout
Chromosome
4
Phenotype
MGI:2446630
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ago1em1flox/Cya mice (Catalog S-CKO-18237) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000239399
NCBI RefSeq
NM_001317173
Target Region
Exon 3~8
Size of Effective Region
~4.1 kb
Detailed Document
Click here to download >>
Overview of Gene Research
AGO1, encoding the Argonaute 1 protein, functions in gene-silencing pathways mediated by small non-coding RNAs. It is involved in various biological processes, such as plant growth and development, and is also crucial in mammalian cell development and metabolism [1,2]. In plants, it physically interacts with HSP90 to buffer genetic variation [2].

In mouse models, EC-AGO1 knockout (KO) leads to an anti-obesity phenotype, with lower body weight, improved insulin sensitivity, and enhanced adipose tissue browning and thermogenesis. Mechanistically, EC-AGO1 suppression inhibits thrombospondin-1 (THBS1), an anti-angiogenic and pro-inflammatory cytokine [3]. In myogenic differentiation, Ago1 is required for activation of the myogenic program by regulating enhancer RNA (eRNA)-CREB-binding protein (CBP) acetyltransferase interaction [4]. In mouse embryonic stem cells (mESCs), Ago1 depletion results in a specific redistribution of the repressive histone mark H3K9me3 and the heterochromatin protein HP1α away from pericentromeric regions, and up-regulation of major satellite transcripts [5].

In conclusion, AGO1 plays essential roles in gene-silencing pathways and is involved in diverse biological processes from plant development to mammalian cell differentiation, metabolism, and chromatin organization. Mouse KO models have revealed its significance in metabolic disorders and cell differentiation, providing insights into potential therapeutic targets for related diseases.

References:
1. Schalk, Audrey, Cousin, Margot A, Dsouza, Nikita R, Piton, Amelie, Gerard, Benedicte. 2021. De novo coding variants in the AGO1 gene cause a neurodevelopmental disorder with intellectual disability. In Journal of medical genetics, 59, 965-975. doi:10.1136/jmedgenet-2021-107751. https://pubmed.ncbi.nlm.nih.gov/34930816/
2. Lemus, Tzitziki, Mason, Grace Alex, Bubb, Kerry L, Queitsch, Christine, Cuperus, Josh T. . AGO1 and HSP90 buffer different genetic variants in Arabidopsis thaliana. In Genetics, 223, . doi:10.1093/genetics/iyac163. https://pubmed.ncbi.nlm.nih.gov/36303325/
3. Tang, Xiaofang, Miao, Yifei, Luo, Yingjun, Zhong, Sheng, Chen, Zhen Bouman. 2020. Suppression of Endothelial AGO1 Promotes Adipose Tissue Browning and Improves Metabolic Dysfunction. In Circulation, 142, 365-379. doi:10.1161/CIRCULATIONAHA.119.041231. https://pubmed.ncbi.nlm.nih.gov/32393053/
4. Fallatah, Bodor, Shuaib, Muhammad, Adroub, Sabir, Lanzuolo, Chiara, Orlando, Valerio. . Ago1 controls myogenic differentiation by regulating eRNA-mediated CBP-guided epigenome reprogramming. In Cell reports, 37, 110066. doi:10.1016/j.celrep.2021.110066. https://pubmed.ncbi.nlm.nih.gov/34852230/
5. Müller, Madlen, Fäh, Tara, Schaefer, Moritz, Ngondo, Richard Patryk, Ciaudo, Constance. 2022. AGO1 regulates pericentromeric regions in mouse embryonic stem cells. In Life science alliance, 5, . doi:10.26508/lsa.202101277. https://pubmed.ncbi.nlm.nih.gov/35236760/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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