C57BL/6JCya-Tada1em1flox/Cya
Common Name:
Tada1-flox
Product ID:
S-CKO-18251
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tada1-flox
Strain ID
CKOCMP-27878-Tada1-B6J-VB
Gene Name
Product ID
S-CKO-18251
Gene Alias
2900026B15Rik; D1Ertd251e; Staf42; Tada1l
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tada1em1flox/Cya mice (Catalog S-CKO-18251) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027846
NCBI RefSeq
NM_030245.3
Target Region
Exon 2~3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Tada1, also known as Hfi1 in Cryptococcus neoformans, is involved in multiple biological processes. It is a part of the core module of the Spt-Ada-Gcn Acetyltransferase (SAGA) complex, which is a conserved co-activator in eukaryotes. Tada1 is important for maintaining the structural integrity of the SAGA complex [5].
In wheat, TaDA1 (a form of Tada1) acts as a conserved negative regulator of kernel size. Overexpression of TaDA1 decreases kernel size and weight, while its down-regulation has the opposite effect. TaDA1-A physically interacts with TaGW2-B, and they have additive effects on kernel weight [2].
In human cells, Tada1 has been associated with cancer-related processes. In a melanoma model, it was identified as a gene whose loss is involved in resistance to vemurafenib through genome-scale CRISPR-Cas9 knockout screening [1]. Also, in colorectal cancer, miR-7702 acts as a tumor suppressor by directly inhibiting TADA1, suppressing cell migration and invasion [3]. In lung squamous cell carcinoma, LINC00511 promotes tumor progression partially by up-regulating TADA1 via targeting miR-150-5p [4]. Moreover, in ovarian cancer cell lines, TADA1 was screened as a novel gene potentially involved in cisplatin resistance using a Genome-scale CRISPR-Cas9 knockout library [6].
In conclusion, Tada1 has diverse functions across different species. In plants like wheat, it regulates kernel size and weight. In humans, it is implicated in cancer-related processes such as drug resistance, cell migration, and invasion. The use of techniques like gene knockout in screening has been crucial in uncovering these roles of Tada1 in different biological contexts.
References:
1. Shalem, Ophir, Sanjana, Neville E, Hartenian, Ella, Doench, John G, Zhang, Feng. 2013. Genome-scale CRISPR-Cas9 knockout screening in human cells. In Science (New York, N.Y.), 343, 84-87. doi:10.1126/science.1247005. https://pubmed.ncbi.nlm.nih.gov/24336571/
2. Liu, Hong, Li, Huifang, Hao, Chenyang, Li, Tian, Zhang, Xueyong. 2019. TaDA1, a conserved negative regulator of kernel size, has an additive effect with TaGW2 in common wheat (Triticum aestivum L.). In Plant biotechnology journal, 18, 1330-1342. doi:10.1111/pbi.13298. https://pubmed.ncbi.nlm.nih.gov/31733093/
3. Liu, Han, Li, Dongdong, Fang, Hua, Ning, Junnan. 2018. Species-specific function of microRNA-7702 in human colorectal cancer cells via targeting TADA1. In American journal of translational research, 10, 2579-2589. doi:. https://pubmed.ncbi.nlm.nih.gov/30210694/
4. Wu, Ying, Li, Li, Wang, Qun, Wang, Xihua, Liu, Hongbing. . LINC00511 promotes lung squamous cell carcinoma proliferation and migration via inhibiting miR-150-5p and activating TADA1. In Translational lung cancer research, 9, 1138-1148. doi:10.21037/tlcr-19-701. https://pubmed.ncbi.nlm.nih.gov/32953492/
5. Yu, Chendi K, Stephenson, Christina J, Villamor, Tristan C, Schulz, Benjamin L, Fraser, James A. 2023. SAGA Complex Subunit Hfi1 Is Important in the Stress Response and Pathogenesis of Cryptococcus neoformans. In Journal of fungi (Basel, Switzerland), 9, . doi:10.3390/jof9121198. https://pubmed.ncbi.nlm.nih.gov/38132798/
6. Ouyang, Qianying, Liu, Yujie, Tan, Jieqiong, Zhou, Honghao, Liu, Yingzi. 2019. Loss of ZNF587B and SULF1 contributed to cisplatin resistance in ovarian cancer cell lines based on Genome-scale Nuclease technology screening. In American journal of cancer research, 9, 988-998. doi:. https://pubmed.ncbi.nlm.nih.gov/31218106/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen