C57BL/6JCya-Snu13em1flox/Cya
Common Name:
Snu13-flox
Product ID:
S-CKO-18261
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Snu13-flox
Strain ID
CKOCMP-20826-Snu13-B6J-VB
Gene Name
Product ID
S-CKO-18261
Gene Alias
FA-1; Fta1; Nhp2l1; Ssfa1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Snu13em1flox/Cya mice (Catalog S-CKO-18261) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000080622
NCBI RefSeq
NM_011482.4
Target Region
Exon 2~3
Size of Effective Region
~3.4 kb
Detailed Document
Overview of Gene Research
Snu13, a key component of the assembling spliceosome, is essential for pre-mRNA splicing, a crucial process in gene expression. It is conserved across species, with its structure being highly similar in the red alga Cyanidioschyzon merolae, yeast, and humans [1]. Snu13 binds specifically to the U14 C/D box snoRNA K-turn sequence motif, which is a prerequisite for the assembly of the ribonucleoprotein (RNP) complex containing NOP58/Nop58, NOP56/Nop56, and the 2'-O-methyl-transferase Fibrillarin/Nop1p [3].
In ovarian cancer, the spliceosomal protein Snu13 promotes therapy resistance. Molecules secreted by ovarian cancer cells upon therapy, which are enriched with spliceosomal components including Snu13, enhance cisplatin resistance and DNA damage repair in recipient cancer cells [2]. In acute ischemic stroke, Snu13 was identified as one of the hub metabolism-related genes. Its expression level is related to the infiltration levels of multiple immune cells, and it may play a role in the molecular mechanisms of acute ischemic stroke [4].
In conclusion, Snu13 is vital for pre-mRNA splicing and RNP assembly. Its role in promoting therapy resistance in ovarian cancer and its association with immune cell infiltration in acute ischemic stroke, as revealed through these studies, highlight its significance in disease-related biological processes. Understanding Snu13's functions may provide new insights into the mechanisms of these diseases and potential therapeutic strategies.
References：
1. Black, C S, Garside, E L, MacMillan, A M, Rader, S D. 2016. Conserved structure of Snu13 from the highly reduced spliceosome of Cyanidioschyzon merolae. In Protein science : a publication of the Protein Society, 25, 911-6. doi:10.1002/pro.2894. https://pubmed.ncbi.nlm.nih.gov/26833716/
2. Shender, Victoria O, Anufrieva, Ksenia S, Shnaider, Polina V, Lagarkova, Maria A, Govorun, Vadim M. 2024. Therapy-induced secretion of spliceosomal components mediates pro-survival crosstalk between ovarian cancer cells. In Nature communications, 15, 5237. doi:10.1038/s41467-024-49512-6. https://pubmed.ncbi.nlm.nih.gov/38898005/
3. Chagot, Marie-Eve, Quinternet, Marc, Rothé, Benjamin, Manival, Xavier, Lebars, Isabelle. 2019. The yeast C/D box snoRNA U14 adopts a "weak" K-turn like conformation recognized by the Snu13 core protein in solution. In Biochimie, 164, 70-82. doi:10.1016/j.biochi.2019.03.014. https://pubmed.ncbi.nlm.nih.gov/30914254/
4. Zhang, Xianjing, Xu, Tengxiao, Wang, Chen, Yue, Maokui, Li, Hao. 2024. Revealing the potential role of hub metabolism-related genes and their correlation with immune cells in acute ischemic stroke. In IET systems biology, 18, 129-142. doi:10.1049/syb2.12095. https://pubmed.ncbi.nlm.nih.gov/38850201/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen