C57BL/6JCya-Sun2em1flox/Cya
Common Name:
Sun2-flox
Product ID:
S-CKO-18267
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Sun2-flox
Strain ID
CKOCMP-223697-Sun2-B6J-VB
Gene Name
Product ID
S-CKO-18267
Gene Alias
B230369L08Rik; C030011B15; Unc84b
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sun2em1flox/Cya mice (Catalog S-CKO-18267) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000046259
NCBI RefSeq
NM_001205345
Target Region
Exon 3~4
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Sun2, also known as SAD1/UNC84 domain protein-2, is a nuclear membrane protein that plays diverse and crucial roles. It is a component of the linker of the nucleoskeleton and cytoskeleton (LINC) complex, which tethers the cell nucleus to the cytoskeleton, regulating mechanical forces during cell migration, differentiation, etc. It also has functions related to maintaining cell nuclear structure, organizing the natural nuclear architecture in cancer cells, and is involved in processes like gene expression regulation [3,6].
In cancer, Sun2 acts as a tumor suppressor. FBXO2, an E3 ubiquitin ligase, targets glycosylated Sun2 for ubiquitination and degradation via the ubiquitin-proteasome system, promoting ovarian cancer development. This discovery of the SOX6-FBXO2-Sun2 axis suggests targeting this axis could be an effective ovarian cancer treatment strategy [1]. In flavivirus replication, Nuclease technology-mediated knockout of Sun2 significantly reduces the replication of Zika virus, dengue virus, and Japanese encephalitis virus, indicating its role in facilitating cytoskeleton rearrangement and viral replication organelle formation [2]. In HSV-1 propagation, disruption of Sun2 leads to increased extracellular viral yields, showing its antiviral property [4]. In response to extracellular matrix rigidity, Sun2 expression is regulated. High Sun2 levels on rigid substrates promote metaphase-to-anaphase transition by acting on mitotic spindle formation, while low levels on soft substrates delay anaphase onset [5].
In conclusion, Sun2 is a multifunctional protein with key roles in cancer suppression, viral replication, and cell division regulation. The gene knockout studies have been instrumental in revealing its functions in these biological processes and disease conditions, providing potential therapeutic targets for cancer and insights into viral-host interactions.
References:
1. Ji, Jing, Shen, Jing, Xu, Yuxin, Liu, Wei, Liu, Bin. 2022. FBXO2 targets glycosylated SUN2 for ubiquitination and degradation to promote ovarian cancer development. In Cell death & disease, 13, 442. doi:10.1038/s41419-022-04892-9. https://pubmed.ncbi.nlm.nih.gov/35525855/
2. Huang, Yanxia, Peng, Qinyu, Tian, Xu, Liu, Chao, Zhang, Ping. 2024. Nuclear membrane protein SUN2 promotes replication of flaviviruses through modulating cytoskeleton reorganization mediated by NS1. In Nature communications, 15, 296. doi:10.1038/s41467-023-44580-6. https://pubmed.ncbi.nlm.nih.gov/38177122/
3. Chen, Xin, Chen, Yu, Huang, Hui-Min, Meng, Xiao-Ming, Li, Jun. 2018. SUN2: A potential therapeutic target in cancer. In Oncology letters, 17, 1401-1408. doi:10.3892/ol.2018.9764. https://pubmed.ncbi.nlm.nih.gov/30675193/
4. Cruz-Palomar, Kendra, Hawkins, Josiane, Vandal, Catherine, Gagnon, Étienne, Lippé, Roger. 2022. SUN2 Modulates the Propagation of HSV-1. In Journal of virology, 96, e0045322. doi:10.1128/jvi.00453-22. https://pubmed.ncbi.nlm.nih.gov/35435724/
5. Belaadi, Nejma, Pernet, Lydia, Aureille, Julien, Loirand, Gervaise, Guilluy, Christophe. 2022. SUN2 regulates mitotic duration in response to extracellular matrix rigidity. In Proceedings of the National Academy of Sciences of the United States of America, 119, e2116167119. doi:10.1073/pnas.2116167119. https://pubmed.ncbi.nlm.nih.gov/36322767/
6. Sharma, Rahul, Hetzer, Martin W. 2023. Disulfide bond in SUN2 regulates dynamic remodeling of LINC complexes at the nuclear envelope. In Life science alliance, 6, . doi:10.26508/lsa.202302031. https://pubmed.ncbi.nlm.nih.gov/37188462/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen