C57BL/6JCya-Tmem43em1flox/Cya
Common Name:
Tmem43-flox
Product ID:
S-CKO-18271
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Tmem43-flox
Strain ID
CKOCMP-74122-Tmem43-B6J-VB
Gene Name
Product ID
S-CKO-18271
Gene Alias
1200015A22Rik; LUMA
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmem43em1flox/Cya mice (Catalog S-CKO-18271) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032183
NCBI RefSeq
NM_028766
Target Region
Exon 5~7
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
TMEM43, also known as LUMA, is a ubiquitously expressed transmembrane protein with phylogenetically high conservation [6]. It has been implicated in multiple biological functions and disease conditions. TMEM43 is associated with pathways related to cardiac function, metabolism, and is involved in diseases such as arrhythmogenic right ventricular dysplasia/cardiomyopathy, Emery-Dreifuss muscular dystrophy, and various cancers [2,3,4,7]. Genetic models, like mouse models, have been crucial in understanding its functions.
In mice, TMEM43 knockdown in hearts deteriorated lipopolysaccharide (LPS)-induced cardiac injury and dysfunction, while overexpression alleviated these effects by inhibiting ferroptosis, suggesting its protective role in sepsis-induced cardiac injury [1]. The TMEM43 S358L mutation in knock-in mouse models led to systolic dysfunction, arrhythmias, fibro-fatty infiltration in the heart, along with abnormal intestinal phenotypes such as elongated villi and fatty infiltration, indicating its importance in maintaining cardiac and intestinal homeostasis [3]. In zebrafish, overexpression of WT and mutant TMEM43 variants led to cardiac morphological and ultrastructural defects, and Nuclease technology mutants showed age-dependent heart enlargement [5].
In conclusion, TMEM43 plays essential roles in maintaining cardiac and intestinal homeostasis, and inhibiting ferroptosis in the context of sepsis-induced cardiac injury. Studies using KO/CKO mouse models and other genetic models have significantly contributed to understanding its functions in arrhythmogenic cardiomyopathy and related cardiac and metabolic diseases, providing insights into potential therapeutic strategies for these conditions.
References:
1. Chen, Zhen, Cao, Zhe, Gui, Feng, Ai, Fen, Zhang, Jun. 2022. TMEM43 Protects against Sepsis-Induced Cardiac Injury via Inhibiting Ferroptosis in Mice. In Cells, 11, . doi:10.3390/cells11192992. https://pubmed.ncbi.nlm.nih.gov/36230956/
2. Heller, Scott A, Shih, Renata, Kalra, Raghav, Kang, Peter B. 2019. Emery-Dreifuss muscular dystrophy. In Muscle & nerve, 61, 436-448. doi:10.1002/mus.26782. https://pubmed.ncbi.nlm.nih.gov/31840275/
3. Orgil, Buyan-Ochir, Munkhsaikhan, Undral, Pierre, Joseph F, Towbin, Jeffrey A, Purevjav, Enkhsaikhan. 2023. The TMEM43 S358L mutation affects cardiac, small intestine, and metabolic homeostasis in a knock-in mouse model. In American journal of physiology. Heart and circulatory physiology, 324, H866-H880. doi:10.1152/ajpheart.00712.2022. https://pubmed.ncbi.nlm.nih.gov/37083466/
4. Zheng, Guoxing, Jiang, Changying, Li, Yulin, Du, Jie, Lin, Xin. 2018. TMEM43-S358L mutation enhances NF-κB-TGFβ signal cascade in arrhythmogenic right ventricular dysplasia/cardiomyopathy. In Protein & cell, 10, 104-119. doi:10.1007/s13238-018-0563-2. https://pubmed.ncbi.nlm.nih.gov/29980933/
5. Zink, Miriam, Seewald, Anne, Rohrbach, Mareike, Childs, Sarah J, Gerull, Brenda. 2022. Altered Expression of TMEM43 Causes Abnormal Cardiac Structure and Function in Zebrafish. In International journal of molecular sciences, 23, . doi:10.3390/ijms23179530. https://pubmed.ncbi.nlm.nih.gov/36076925/
6. Ratnavadivel, Sandra, Dammeier, Joline, Gaertner, Anna, Paululat, Achim, Milting, Hendrik. 2024. Generation of a TMEM43 knockout human induced pluripotent stem cell line (HDZi003-A-1) using Nuclease technology. In Stem cell research, 76, 103354. doi:10.1016/j.scr.2024.103354. https://pubmed.ncbi.nlm.nih.gov/38430734/
7. Gu, Qingqing, Xu, Fuyi, Orgil, Buyan-Ochir, Purevjav, Enkhsaikhan, Lu, Lu. 2021. Systems genetics analysis defines importance of TMEM43/LUMA for cardiac- and metabolic-related pathways. In Physiological genomics, 54, 22-35. doi:10.1152/physiolgenomics.00066.2021. https://pubmed.ncbi.nlm.nih.gov/34766515/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen