C57BL/6JCya-Cyp26b1em1flox/Cya
Common Name:
Cyp26b1-flox
Product ID:
S-CKO-18294
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Cyp26b1-flox
Strain ID
CKOCMP-232174-Cyp26b1-B6J-VA
Gene Name
Product ID
S-CKO-18294
Gene Alias
CP26; P450RAI-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cyp26b1em1flox/Cya mice (Catalog S-CKO-18294) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000168003
NCBI RefSeq
NM_001177713
Target Region
Exon 4~7
Size of Effective Region
~3.8 kb
Detailed Document
Overview of Gene Research
Cyp26b1, a member of the cytochrome P450 family, is crucial for breaking down retinoic acid. Maintaining appropriate retinoic acid levels is essential for normal development of the cardiovascular and lymphatic systems, as well as other biological processes such as meiosis in the testis, lung development, and epidermal keratinization [1,4,5,2].
Genetic ablation of Cyp26b1 in mouse embryos leads to abnormally thickened aortic valve leaflets due to increased endothelial and mesenchymal cell proliferation, and also causes ventricular septal defects in some null embryos, indicating its role in heart valve morphogenesis [3]. In the lung, loss of Cyp26b1 results in reduction of alveolar type 1 cells, failure of alveolar inflation, and early postnatal lethality, along with expansion of distal epithelial progenitors [5].
In conclusion, Cyp26b1 is an essential regulator in multiple biological processes, especially in the development of the cardiovascular, lymphatic, and respiratory systems. The gene knockout mouse models have revealed its critical roles in these processes, and also provided insights into related diseases such as lymphedema, heart valve disorders, and keratinization disorders [1,3,2].
References：
1. Ricci, M, Serrani, R, Amato, B, Basha, S H, Bertelli, M. . CYP26B1 and its implications in lymphangiogenesis: Literature review and study of rare variants in two families. In Lymphology, 53, 20-28. doi:. https://pubmed.ncbi.nlm.nih.gov/32521127/
2. Veit, Joachim G S, De Glas, Valérie, Balau, Benoît, Poumay, Yves, Diaz, Philippe. 2020. Characterization of CYP26B1-Selective Inhibitor, DX314, as a Potential Therapeutic for Keratinization Disorders. In The Journal of investigative dermatology, 141, 72-83.e6. doi:10.1016/j.jid.2020.05.090. https://pubmed.ncbi.nlm.nih.gov/32505549/
3. Ahuja, Neha, Hiltabidle, Max S, Rajasekhar, Hariprem, Cleaver, Ondine, Maynard, Caitlin. 2022. Endothelial Cyp26b1 restrains murine heart valve growth during development. In Developmental biology, 486, 81-95. doi:10.1016/j.ydbio.2022.03.003. https://pubmed.ncbi.nlm.nih.gov/35364055/
4. Edelsztein, Nadia Y, Kashimada, Kenichi, Schteingart, Helena F, Rey, Rodolfo A. 2019. CYP26B1 declines postnatally in Sertoli cells independently of androgen action in the mouse testis. In Molecular reproduction and development, 87, 66-77. doi:10.1002/mrd.23302. https://pubmed.ncbi.nlm.nih.gov/31755607/
5. Daniel, Edward, Barlow, Haley R, Sutton, Gabrielle I, Cowdin, Mitzy A, Cleaver, Ondine. 2020. Cyp26b1 is an essential regulator of distal airway epithelial differentiation during lung development. In Development (Cambridge, England), 147, . doi:10.1242/dev.181560. https://pubmed.ncbi.nlm.nih.gov/32001436/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen