C57BL/6JCya-Gldcem1flox/Cya
Common Name
Gldc-flox
Product ID
S-CKO-18297
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-104174-Gldc-B6J-VB
Status
When using this mouse strain in a publication, please cite “Gldc-flox Mouse (Catalog S-CKO-18297) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Gldc-flox
Strain ID
CKOCMP-104174-Gldc-B6J-VB
Gene Name
Product ID
S-CKO-18297
Gene Alias
D19Wsu57e, b2b2679Clo, D030049L12Rik
Background
C57BL/6JCya
Gene Full Name
glycine decarboxylase
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 19 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000025778
NCBI Transcript ID
NM_138595.2
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Overview of Gene Research
Gldc, or glycine decarboxylase, is a core enzyme in the glycine cleavage system located on the mitochondrial membrane. It is responsible for regulating glycine levels, contributing one-carbon units to folate metabolism, and is crucial for normal development and metabolism [3].
Mutations in Gldc are the main cause of classic nonketotic hyperglycinemia (NKH), with 80% of subjects in a study having Gldc mutations [1]. In a zebrafish model of Gldc deficiency, embryos showed renal, craniofacial, and neural development defects, indicating its essential role in renal progenitor patterning during kidney development [3].
In cancer research, in prostate cancer, Gldc plays a central role in glycolysis, enhances aerobic glycolysis, and promotes cell migration, invasion, and immune escape [2]. In TNBC, Gldc is up-regulated, promotes cell proliferation, and is correlated with tumor immune infiltration [4]. In colorectal cancer, Gldc promotes metastasis through the Hippo signaling pathway-mediated epithelial-mesenchymal transition [5].
In conclusion, Gldc is essential for regulating glycine metabolism and has a significant impact on development and disease. Its role in NKH and various cancers, as revealed by genetic models such as zebrafish and through studies in human subjects, provides valuable insights into potential therapeutic targets for these diseases.
References：
1. Coughlin, Curtis R, Swanson, Michael A, Kronquist, Kathryn, Scharer, Gunter H, Van Hove, Johan L K. 2016. The genetic basis of classic nonketotic hyperglycinemia due to mutations in GLDC and AMT. In Genetics in medicine : official journal of the American College of Medical Genetics, 19, 104-111. doi:10.1038/gim.2016.74. https://pubmed.ncbi.nlm.nih.gov/27362913/
2. Chen, Ming-Kun, Xiao, Zhuo-Yu, Huang, Zhi-Peng, Liu, Yang, Zhao, Shan-Chao. 2023. Glycine Decarboxylase (GLDC) Plays a Crucial Role in Regulating Energy Metabolism, Invasion, Metastasis and Immune Escape for Prostate Cancer. In International journal of biological sciences, 19, 4726-4743. doi:10.7150/ijbs.85893. https://pubmed.ncbi.nlm.nih.gov/37781511/
3. Weaver, Nicole E, Healy, Allison, Wingert, Rebecca A. 2022. gldc Is Essential for Renal Progenitor Patterning during Kidney Development. In Biomedicines, 10, . doi:10.3390/biomedicines10123220. https://pubmed.ncbi.nlm.nih.gov/36551976/
4. Xie, Huaying, Yan, Tingting, Lu, Xinxin, Zhou, Liheng, Ma, Jun. 2022. GLDC mitigated by miR-30e regulates cell proliferation and tumor immune infiltration in TNBC. In Frontiers in immunology, 13, 1033367. doi:10.3389/fimmu.2022.1033367. https://pubmed.ncbi.nlm.nih.gov/36275705/
5. Yu, Hao, Hu, Xueqing, Zhang, Yingru, Wang, Yan, Zhu, Huirong. 2023. GLDC promotes colorectal cancer metastasis through epithelial-mesenchymal transition mediated by Hippo signaling pathway. In Medical oncology (Northwood, London, England), 40, 293. doi:10.1007/s12032-023-02076-9. https://pubmed.ncbi.nlm.nih.gov/37668829/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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