C57BL/6NCya-Msl1em1flox/Cya
Common Name
Msl1-flox
Product ID
S-CKO-18299
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-74026-Msl1-B6N-VA
Status
When using this mouse strain in a publication, please cite “Msl1-flox Mouse (Catalog S-CKO-18299) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Msl1-flox
Strain ID
CKOCMP-74026-Msl1-B6N-VA
Gene Name
Product ID
S-CKO-18299
Gene Alias
Msl-1, 2810017F12Rik, 4121402D02Rik, 4930463F05Rik
Background
C57BL/6NCya
Gene Full Name
male specific lethal 1
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 11 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000037915
NCBI Transcript ID
NM_028722
Target Region
Exon 3
Size of Effective Region
~1.9 kb
Overview of Gene Research
Msl1, or Male-specific lethal 1, is crucial for the formation of the MSL histone acetyltransferase complex. This complex acetylates histone H4 Lys16 (H4K16ac), a key epigenetic modification that activates gene expression. It is involved in multiple biological processes and associated with pathways like dosage compensation in Drosophila and potentially in mammalian cells as well, which is important for balanced gene expression between sexes [1,2,3,4].
In a mouse model, Msl1 was identified as a key regulator of STAT3 and histone H4 in hepatocytes. It forms condensates with STAT3 or H4 through liquid-liquid phase separation to enrich acetyl-coenzyme A (Ac-CoA). Ac-CoA then enhances Msl1 condensate formation, promoting the acetylation of STAT3 K685 and H4K16, thus stimulating liver regeneration after partial hepatectomy (PH). This shows Msl1's role in liver regeneration [1].
In conclusion, Msl1 is essential for gene activation through histone acetylation and has a significant role in liver regeneration as revealed by in vivo studies. Understanding Msl1's function in such processes may offer new therapeutic strategies for acute liver diseases and transplantation [1].
References：
1. He, Yucheng, Wang, Shichao, Liu, Shenghui, Chen, Xiangmei, Zhang, Lisheng. 2023. MSL1 Promotes Liver Regeneration by Driving Phase Separation of STAT3 and Histone H4 and Enhancing Their Acetylation. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2301094. doi:10.1002/advs.202301094. https://pubmed.ncbi.nlm.nih.gov/37279389/
2. Hallacli, Erinc, Lipp, Michael, Georgiev, Plamen, Akhtar, Asifa, Kadlec, Jan. 2012. Msl1-mediated dimerization of the dosage compensation complex is essential for male X-chromosome regulation in Drosophila. In Molecular cell, 48, 587-600. doi:10.1016/j.molcel.2012.09.014. https://pubmed.ncbi.nlm.nih.gov/23084835/
3. Babosha, Valentin, Klimenko, Natalia, Revel-Muroz, Anastasia, Georgiev, Pavel, Maksimenko, Oksana. 2024. N-terminus of Drosophila melanogaster MSL1 is critical for dosage compensation. In eLife, 13, . doi:10.7554/eLife.93241. https://pubmed.ncbi.nlm.nih.gov/39699942/
4. Chlamydas, Sarantis, Holz, Herbert, Samata, Maria, Steinmetz, Lars M, Akhtar, Asifa. 2016. Functional interplay between MSL1 and CDK7 controls RNA polymerase II Ser5 phosphorylation. In Nature structural & molecular biology, 23, 580-9. doi:10.1038/nsmb.3233. https://pubmed.ncbi.nlm.nih.gov/27183194/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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