C57BL/6JCya-Acsm5em1flox/Cya
Common Name:
Acsm5-flox
Product ID:
S-CKO-18311
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Acsm5-flox
Strain ID
CKOCMP-272428-Acsm5-B6J-VB
Gene Name
Product ID
S-CKO-18311
Gene Alias
C730019D22; C730027J19Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Acsm5em1flox/Cya mice (Catalog S-CKO-18311) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000066465
NCBI RefSeq
NM_178758
Target Region
Exon 3~4
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
Acsm5, acyl-CoA synthetase medium-chain family member 5, is involved in fatty acid metabolism. It activates medium-chain fatty acids by converting them to acyl-CoA esters, which are important for processes like β-oxidation and lipid synthesis. This gene is associated with multiple biological pathways, and its dysregulation can have implications for various physiological and pathological conditions [2,4,5].
In the context of diseases, in ligamentum flavum (LF) hypertrophy, Acsm5 is significantly down-regulated. Overexpression of Acsm5 in LF cells in vitro inhibits free fatty acid (FFA)-induced lipid accumulation and fibrosis. In vivo in mice, it also inhibits LF thickening, lipid accumulation, and fibrosis, acting through the inhibition of the FABP4-mediated PPARγ signaling pathway [1]. In hepatocellular carcinoma (HCC), Acsm5 is down-regulated, and its overexpression in HCC cell lines reduces fatty acid accumulation, cell proliferation, migration, and invasion in vitro and inhibits tumor growth in mouse xenografts. DNA methylation by DNMT1 mediates the down-regulation of Acsm5 in this context [2]. In lumbar ligamentum flavum hypertrophy, hypermethylation of Acsm5, mediated by DNMT1, promotes the proliferation and fibrosis and inhibits the apoptosis of ligamentum flavum cells [3].
In conclusion, Acsm5 is crucial in regulating fatty acid metabolism. Through model-based research, especially in KO/CKO mouse models and in vitro cell experiments, it has been shown to play important roles in diseases such as LF hypertrophy and HCC. Understanding Acsm5 provides insights into the underlying mechanisms of these diseases, potentially leading to new therapeutic strategies targeting lipid metabolism and related pathways.
References:
1. Cao, Yanlin, Li, Jianjun, Qiu, Sujun, Ni, Songjia, Duan, Yang. 2023. ACSM5 inhibits ligamentum flavum hypertrophy by regulating lipid accumulation mediated by FABP4/PPAR signaling pathway. In Biology direct, 18, 75. doi:10.1186/s13062-023-00436-z. https://pubmed.ncbi.nlm.nih.gov/37957699/
2. Yang, Lei, Pham, Kien, Xi, Yibo, Robertson, Keith D, Liu, Chen. 2024. Acyl-CoA Synthetase Medium-Chain Family Member 5-Mediated Fatty Acid Metabolism Dysregulation Promotes the Progression of Hepatocellular Carcinoma. In The American journal of pathology, 194, 1951-1966. doi:10.1016/j.ajpath.2024.07.002. https://pubmed.ncbi.nlm.nih.gov/39069168/
3. Cao, Yanlin, Zhan, Yenan, Qiu, Sujun, Ni, Songjia, Duan, Yang. 2021. Integrative analysis of genome-wide DNA methylation and single-nucleotide polymorphism identified ACSM5 as a suppressor of lumbar ligamentum flavum hypertrophy. In Arthritis research & therapy, 23, 251. doi:10.1186/s13075-021-02625-5. https://pubmed.ncbi.nlm.nih.gov/34593020/
4. Rinaldi, Anna, Lazareth, Hélène, Poindessous, Virginie, Cippà, Pietro E, Pallet, Nicolas. 2022. Impaired fatty acid metabolism perpetuates lipotoxicity along the transition to chronic kidney injury. In JCI insight, 7, . doi:10.1172/jci.insight.161783. https://pubmed.ncbi.nlm.nih.gov/35998043/
5. Mizuno, Yumi, Tamaru, Shunsuke, Tochigi, Hideno, Ishihara, Osamu, Kajihara, Takeshi. 2024. Decidualized Endometrial Stromal Cells Promote Mitochondrial Beta-Oxidation to Produce the Octanoic Acid Required for Implantation. In Biomolecules, 14, . doi:10.3390/biom14081014. https://pubmed.ncbi.nlm.nih.gov/39199401/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen