C57BL/6JCya-Crls1em1flox/Cya
Common Name:
Crls1-flox
Product ID:
S-CKO-18339
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Crls1-flox
Strain ID
CKOCMP-66586-Crls1-B6J-VB
Gene Name
Product ID
S-CKO-18339
Gene Alias
0610009I22Rik; 4930557M15Rik; 5730490M08Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Crls1em1flox/Cya mice (Catalog S-CKO-18339) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028835
NCBI RefSeq
NM_001024385
Target Region
Exon 4~6
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Crls1, or cardiolipin synthase 1, is crucial for mitochondrial cardiolipin biosynthesis, regulating phosphatidylglycerol remodeling [3,4]. Cardiolipin is essential for mitochondrial structure and function, and thus Crls1 is involved in maintaining mitochondrial integrity, oxidative phosphorylation (OXPHOS), and mitochondrial biogenesis [2,3]. It may also be associated with lipid metabolism-related pathways [4,5]. Genetic models, such as knockout mouse models, are valuable for studying its functions.
Genetic ablation of Crls1 suppresses canonical and noncanonical inflammasome-mediated mitochondrial damage, pyroptosis, and inflammatory cytokine release, indicating its role in the gasdermin D-related pyroptosis pathway [1]. In a mouse model of cardiotoxin-induced muscle damage, AAV9-shCrls1-mediated downregulation of Crls1 impairs muscle regeneration, while AAV9-mCrls1-mediated overexpression improves it, highlighting its significance in age-related muscle deterioration and myogenesis [2]. Hepatocyte-specific Crls1-knockout (Crls1-HKO) mice show exacerbated insulin resistance, hepatic steatosis, inflammation, and fibrosis during non-alcoholic steatohepatitis (NASH) development, suggesting Crls1 ameliorates NASH by inhibiting the expression and activity of activating transcription factor 3 (ATF3) [4].
In conclusion, Crls1 is essential for maintaining mitochondrial function and integrity, and its role extends to processes like pyroptosis, muscle regeneration, and NASH development. The use of Crls1 knockout and conditional knockout mouse models has provided key insights into its functions in these disease-related biological processes, contributing to a better understanding of the underlying mechanisms and potentially paving the way for novel therapeutic strategies.
References:
1. Miao, Rui, Jiang, Cong, Chang, Winston Y, Liu, Xing, Lieberman, Judy. 2023. Gasdermin D permeabilization of mitochondrial inner and outer membranes accelerates and enhances pyroptosis. In Immunity, 56, 2523-2541.e8. doi:10.1016/j.immuni.2023.10.004. https://pubmed.ncbi.nlm.nih.gov/37924812/
2. Yoo, Youngbum, Yeon, MyeongHoon, Kim, Won-Kyung, Ro, Hyunju, Seo, Young-Kyo. 2024. Age-dependent loss of Crls1 causes myopathy and skeletal muscle regeneration failure. In Experimental & molecular medicine, 56, 922-934. doi:10.1038/s12276-024-01199-x. https://pubmed.ncbi.nlm.nih.gov/38556544/
3. Lee, Richard G, Balasubramaniam, Shanti, Stentenbach, Maike, Reid, Gavin E, Filipovska, Aleksandra. . Deleterious variants in CRLS1 lead to cardiolipin deficiency and cause an autosomal recessive multi-system mitochondrial disease. In Human molecular genetics, 31, 3597-3612. doi:10.1093/hmg/ddac040. https://pubmed.ncbi.nlm.nih.gov/35147173/
4. Tu, Chuyue, Xiong, Hui, Hu, Yufeng, Zhang, Peng, Mei, Zhinan. 2020. Cardiolipin Synthase 1 Ameliorates NASH Through Activating Transcription Factor 3 Transcriptional Inactivation. In Hepatology (Baltimore, Md.), 72, 1949-1967. doi:10.1002/hep.31202. https://pubmed.ncbi.nlm.nih.gov/32096565/
5. Feng, Hai-Ming, Zhao, Ye, Zhang, Jian-Ping, Li, Bin, Wang, Cheng. 2017. Expression and potential mechanism of metabolism-related genes and CRLS1 in non-small cell lung cancer. In Oncology letters, 15, 2661-2668. doi:10.3892/ol.2017.7591. https://pubmed.ncbi.nlm.nih.gov/29434989/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen