C57BL/6JCya-Rev1em1flox/Cya
Common Name:
Rev1-flox
Product ID:
S-CKO-18366
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rev1-flox
Strain ID
CKOCMP-56210-Rev1-B6J-VB
Gene Name
Product ID
S-CKO-18366
Gene Alias
1110027I23Rik; Rev1l
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rev1em1flox/Cya mice (Catalog S-CKO-18366) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027251
NCBI RefSeq
NM_019570
Target Region
Exon 10~11
Size of Effective Region
~4.8 kb
Detailed Document
Overview of Gene Research
Rev1, a member of the Y family polymerases, plays a key role in the translesion synthesis (TLS) pathway. TLS is an error-prone process that enables cells to overcome replication blockage caused by DNA damage, safeguarding genome integrity and cell survival [1,4].
Rev1-mutated mouse models, such as the Rev1 transgenic (Tg) mouse, have been used to study its role in mutagenesis and carcinogenesis. In Rev1-Tg mice treated with N-methyl-N-nitrosourea (MNU), the mutation frequency in thymic lymphoma (TL) was higher compared to wild-type (WT) mice, and pre-leukemic cells were detected earlier in Rev1-Tg mice. This indicates that Rev1 overexpression accelerates mutagenesis and MNU-induced TL [3]. In lung cancer, REV1 silencing decreased the growth and proliferation of lung cancer cells, and a novel inhibitor JH-RE-06 suppressed lung tumorigenesis, suggesting REV1 as a potential diagnostic marker and therapeutic target [2].
In conclusion, Rev1 is crucial in the TLS pathway, influencing mutagenesis and carcinogenesis. Mouse models, especially Rev1-Tg, have provided insights into its role in thymic lymphoma and lung cancer, highlighting its significance in understanding tumorigenesis mechanisms and potentially developing cancer therapies.
References:
1. Rizzo, Alessandro A, Korzhnev, Dmitry M. 2019. The Rev1-Polζ translesion synthesis mutasome: Structure, interactions and inhibition. In The Enzymes, 45, 139-181. doi:10.1016/bs.enz.2019.07.001. https://pubmed.ncbi.nlm.nih.gov/31627876/
2. Chen, Yunshang, Jie, Xiaohua, Xing, Biyuan, Li, Zhenyu, Wu, Gang. 2022. REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis. In Cell death & disease, 13, 110. doi:10.1038/s41419-022-04567-5. https://pubmed.ncbi.nlm.nih.gov/35115490/
3. Sasatani, Megumi, Xi, Yang, Daino, Kazuhiro, Sotomaru, Yusuke, Kamiya, Kenji. 2024. Rev1 overexpression accelerates N-methyl-N-nitrosourea (MNU)-induced thymic lymphoma by increasing mutagenesis. In Cancer science, 115, 1808-1819. doi:10.1111/cas.16159. https://pubmed.ncbi.nlm.nih.gov/38572512/
4. Sharma, Shilpy, Canman, Christine E. 2012. REV1 and DNA polymerase zeta in DNA interstrand crosslink repair. In Environmental and molecular mutagenesis, 53, 725-40. doi:10.1002/em.21736. https://pubmed.ncbi.nlm.nih.gov/23065650/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen