C57BL/6JCya-Rev1em1flox/Cya
Common Name
Rev1-flox
Product ID
S-CKO-18366
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-56210-Rev1-B6J-VB
When using this mouse strain in a publication, please cite “Rev1-flox Mouse (Catalog S-CKO-18366) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Rev1-flox
Strain ID
CKOCMP-56210-Rev1-B6J-VB
Gene Name
Product ID
S-CKO-18366
Gene Alias
1110027I23Rik, Rev1l
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000027251
NCBI RefSeq
NM_019570
Target Region
Exon 10~11
Size of Effective Region
~4.8 kb
Overview of Gene Research
Rev1, a member of the Y family polymerases, plays a key role in the translesion synthesis (TLS) pathway. TLS is an error-prone process that enables cells to overcome replication blockage caused by DNA damage, safeguarding genome integrity and cell survival [1,4].
Rev1-mutated mouse models, such as the Rev1 transgenic (Tg) mouse, have been used to study its role in mutagenesis and carcinogenesis. In Rev1-Tg mice treated with N-methyl-N-nitrosourea (MNU), the mutation frequency in thymic lymphoma (TL) was higher compared to wild-type (WT) mice, and pre-leukemic cells were detected earlier in Rev1-Tg mice. This indicates that Rev1 overexpression accelerates mutagenesis and MNU-induced TL [3]. In lung cancer, REV1 silencing decreased the growth and proliferation of lung cancer cells, and a novel inhibitor JH-RE-06 suppressed lung tumorigenesis, suggesting REV1 as a potential diagnostic marker and therapeutic target [2].
In conclusion, Rev1 is crucial in the TLS pathway, influencing mutagenesis and carcinogenesis. Mouse models, especially Rev1-Tg, have provided insights into its role in thymic lymphoma and lung cancer, highlighting its significance in understanding tumorigenesis mechanisms and potentially developing cancer therapies.
References：
1. Rizzo, Alessandro A, Korzhnev, Dmitry M. 2019. The Rev1-Polζ translesion synthesis mutasome: Structure, interactions and inhibition. In The Enzymes, 45, 139-181. doi:10.1016/bs.enz.2019.07.001. https://pubmed.ncbi.nlm.nih.gov/31627876/
2. Chen, Yunshang, Jie, Xiaohua, Xing, Biyuan, Li, Zhenyu, Wu, Gang. 2022. REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis. In Cell death & disease, 13, 110. doi:10.1038/s41419-022-04567-5. https://pubmed.ncbi.nlm.nih.gov/35115490/
3. Sasatani, Megumi, Xi, Yang, Daino, Kazuhiro, Sotomaru, Yusuke, Kamiya, Kenji. 2024. Rev1 overexpression accelerates N-methyl-N-nitrosourea (MNU)-induced thymic lymphoma by increasing mutagenesis. In Cancer science, 115, 1808-1819. doi:10.1111/cas.16159. https://pubmed.ncbi.nlm.nih.gov/38572512/
4. Sharma, Shilpy, Canman, Christine E. 2012. REV1 and DNA polymerase zeta in DNA interstrand crosslink repair. In Environmental and molecular mutagenesis, 53, 725-40. doi:10.1002/em.21736. https://pubmed.ncbi.nlm.nih.gov/23065650/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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