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C57BL/6JCya-Marchf6em1flox/Cya
Common Name:
Marchf6-flox
Product ID:
S-CKO-18377
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Marchf6-flox
Strain ID
CKOCMP-223455-Marchf6-B6J-VB
Gene Name
Marchf6
Product ID
S-CKO-18377
Gene Alias
3830408G03; F830029L24Rik; March6; mKIAA0597
Background
C57BL/6JCya
NCBI ID
223455
Modification
Conditional knockout
Chromosome
15
Phenotype
MGI:2442773
Document
Click here to download >>
Application
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More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Marchf6em1flox/Cya mice (Catalog S-CKO-18377) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000090227
NCBI RefSeq
NM_172606
Target Region
Exon 5
Size of Effective Region
~1.5 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Marchf6, a transmembrane endoplasmic reticulum E3 ubiquitin ligase, is a crucial regulator in multiple biological processes [1,2,3,4,5,6,7]. It participates in endoplasmic reticulum associated degradation, and many of its substrates are involved in sterol and lipid metabolism [3]. Marchf6 also acts as an NADPH sensor, regulating ferroptosis, a form of cell death caused by excessive iron-dependent lipid peroxidation [1,4,5,8].

POMC-Cre-mediated Marchf6-deficient mice exhibit hyperphagia, reduced energy expenditure, and weight gain [2]. This indicates that Marchf6 is essential for regulating endoplasmic reticulum stress, ferroptosis, and metabolic homeostasis in POMC neurons. In addition, inhibiting ferroptosis rescued the growth of MARCHF6-deficient tumours and the peri-natal lethality of Marchf6-/-mice [1].

In conclusion, Marchf6 is a key regulator in multiple biological pathways, including ferroptosis and metabolic homeostasis. The use of Marchf6 KO/CKO mouse models has revealed its important roles in disease-related processes such as tumour growth, perinatal survival, and metabolic disorders. Understanding Marchf6 may provide new insights into the mechanisms of these diseases and potential treatment strategies.

References:
1. Nguyen, Kha The, Mun, Sang-Hyeon, Yang, Jihye, Lee, Yoontae, Hwang, Cheol-Sang. 2022. The MARCHF6 E3 ubiquitin ligase acts as an NADPH sensor for the regulation of ferroptosis. In Nature cell biology, 24, 1239-1251. doi:10.1038/s41556-022-00973-1. https://pubmed.ncbi.nlm.nih.gov/35941365/
2. Mun, Sang-Hyeon, Lee, Chang-Seok, Kim, Hyun Jin, Seong, Je Kyung, Hwang, Cheol-Sang. 2023. Marchf6 E3 ubiquitin ligase critically regulates endoplasmic reticulum stress, ferroptosis, and metabolic homeostasis in POMC neurons. In Cell reports, 42, 112746. doi:10.1016/j.celrep.2023.112746. https://pubmed.ncbi.nlm.nih.gov/37421621/
3. Scott, Nicola A, Sharpe, Laura J, Brown, Andrew J. 2020. The E3 ubiquitin ligase MARCHF6 as a metabolic integrator in cholesterol synthesis and beyond. In Biochimica et biophysica acta. Molecular and cell biology of lipids, 1866, 158837. doi:10.1016/j.bbalip.2020.158837. https://pubmed.ncbi.nlm.nih.gov/33049405/
4. Yang, Jihye, Lee, Yoontae, Hwang, Cheol-Sang. 2023. The ubiquitin-proteasome system links NADPH metabolism to ferroptosis. In Trends in cell biology, 33, 1088-1103. doi:10.1016/j.tcb.2023.07.003. https://pubmed.ncbi.nlm.nih.gov/37558595/
5. Fenton, Nicole M, Qian, Lydia, Paine, Eloise G, Sharpe, Laura J, Brown, Andrew J. 2024. A paREDOX in the control of cholesterol biosynthesis: Does the NADPH sensor and E3 ubiquitin ligase MARCHF6 protect mammalian cells during oxidative stress by controlling sterol biosynthesis? In BioEssays : news and reviews in molecular, cellular and developmental biology, 46, e2400073. doi:10.1002/bies.202400073. https://pubmed.ncbi.nlm.nih.gov/38760877/
6. Yang, Jihye, Kim, Sang-Yoon, Hwang, Cheol-Sang. 2024. Delineation of the substrate recognition domain of MARCHF6 E3 ubiquitin ligase in the Ac/N-degron pathway and its regulatory role in ferroptosis. In The Journal of biological chemistry, 300, 107731. doi:10.1016/j.jbc.2024.107731. https://pubmed.ncbi.nlm.nih.gov/39216628/
7. Qian, Lydia, Scott, Nicola A, Capell-Hattam, Isabelle M, Sharpe, Laura J, Brown, Andrew J. 2023. Cholesterol synthesis enzyme SC4MOL is fine-tuned by sterols and targeted for degradation by the E3 ligase MARCHF6. In Journal of lipid research, 64, 100362. doi:10.1016/j.jlr.2023.100362. https://pubmed.ncbi.nlm.nih.gov/36958722/
8. Kuang, Guixing, Wang, Weidong, Xiong, Dan, Zeng, Chong. 2022. An NADPH sensor that regulates cell ferroptosis. In Journal of translational medicine, 20, 474. doi:10.1186/s12967-022-03658-3. https://pubmed.ncbi.nlm.nih.gov/36266653/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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