C57BL/6JCya-Marchf6em1flox/Cya
Common Name
Marchf6-flox
Product ID
S-CKO-18377
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-223455-Marchf6-B6J-VB
When using this mouse strain in a publication, please cite “Marchf6-flox Mouse (Catalog S-CKO-18377) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Marchf6-flox
Strain ID
CKOCMP-223455-Marchf6-B6J-VB
Gene Name
Product ID
S-CKO-18377
Gene Alias
3830408G03, F830029L24Rik, March6, mKIAA0597
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 15
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000090227
NCBI RefSeq
NM_172606
Target Region
Exon 5
Size of Effective Region
~1.5 kb
Overview of Gene Research
Marchf6, a transmembrane endoplasmic reticulum E3 ubiquitin ligase, is a crucial regulator in multiple biological processes [1,2,3,4,5,6,7]. It participates in endoplasmic reticulum associated degradation, and many of its substrates are involved in sterol and lipid metabolism [3]. Marchf6 also acts as an NADPH sensor, regulating ferroptosis, a form of cell death caused by excessive iron-dependent lipid peroxidation [1,4,5,8].
POMC-Cre-mediated Marchf6-deficient mice exhibit hyperphagia, reduced energy expenditure, and weight gain [2]. This indicates that Marchf6 is essential for regulating endoplasmic reticulum stress, ferroptosis, and metabolic homeostasis in POMC neurons. In addition, inhibiting ferroptosis rescued the growth of MARCHF6-deficient tumours and the peri-natal lethality of Marchf6-/-mice [1].
In conclusion, Marchf6 is a key regulator in multiple biological pathways, including ferroptosis and metabolic homeostasis. The use of Marchf6 KO/CKO mouse models has revealed its important roles in disease-related processes such as tumour growth, perinatal survival, and metabolic disorders. Understanding Marchf6 may provide new insights into the mechanisms of these diseases and potential treatment strategies.
References：
1. Nguyen, Kha The, Mun, Sang-Hyeon, Yang, Jihye, Lee, Yoontae, Hwang, Cheol-Sang. 2022. The MARCHF6 E3 ubiquitin ligase acts as an NADPH sensor for the regulation of ferroptosis. In Nature cell biology, 24, 1239-1251. doi:10.1038/s41556-022-00973-1. https://pubmed.ncbi.nlm.nih.gov/35941365/
2. Mun, Sang-Hyeon, Lee, Chang-Seok, Kim, Hyun Jin, Seong, Je Kyung, Hwang, Cheol-Sang. 2023. Marchf6 E3 ubiquitin ligase critically regulates endoplasmic reticulum stress, ferroptosis, and metabolic homeostasis in POMC neurons. In Cell reports, 42, 112746. doi:10.1016/j.celrep.2023.112746. https://pubmed.ncbi.nlm.nih.gov/37421621/
3. Scott, Nicola A, Sharpe, Laura J, Brown, Andrew J. 2020. The E3 ubiquitin ligase MARCHF6 as a metabolic integrator in cholesterol synthesis and beyond. In Biochimica et biophysica acta. Molecular and cell biology of lipids, 1866, 158837. doi:10.1016/j.bbalip.2020.158837. https://pubmed.ncbi.nlm.nih.gov/33049405/
4. Yang, Jihye, Lee, Yoontae, Hwang, Cheol-Sang. 2023. The ubiquitin-proteasome system links NADPH metabolism to ferroptosis. In Trends in cell biology, 33, 1088-1103. doi:10.1016/j.tcb.2023.07.003. https://pubmed.ncbi.nlm.nih.gov/37558595/
5. Fenton, Nicole M, Qian, Lydia, Paine, Eloise G, Sharpe, Laura J, Brown, Andrew J. 2024. A paREDOX in the control of cholesterol biosynthesis: Does the NADPH sensor and E3 ubiquitin ligase MARCHF6 protect mammalian cells during oxidative stress by controlling sterol biosynthesis? In BioEssays : news and reviews in molecular, cellular and developmental biology, 46, e2400073. doi:10.1002/bies.202400073. https://pubmed.ncbi.nlm.nih.gov/38760877/
6. Yang, Jihye, Kim, Sang-Yoon, Hwang, Cheol-Sang. 2024. Delineation of the substrate recognition domain of MARCHF6 E3 ubiquitin ligase in the Ac/N-degron pathway and its regulatory role in ferroptosis. In The Journal of biological chemistry, 300, 107731. doi:10.1016/j.jbc.2024.107731. https://pubmed.ncbi.nlm.nih.gov/39216628/
7. Qian, Lydia, Scott, Nicola A, Capell-Hattam, Isabelle M, Sharpe, Laura J, Brown, Andrew J. 2023. Cholesterol synthesis enzyme SC4MOL is fine-tuned by sterols and targeted for degradation by the E3 ligase MARCHF6. In Journal of lipid research, 64, 100362. doi:10.1016/j.jlr.2023.100362. https://pubmed.ncbi.nlm.nih.gov/36958722/
8. Kuang, Guixing, Wang, Weidong, Xiong, Dan, Zeng, Chong. 2022. An NADPH sensor that regulates cell ferroptosis. In Journal of translational medicine, 20, 474. doi:10.1186/s12967-022-03658-3. https://pubmed.ncbi.nlm.nih.gov/36266653/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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