C57BL/6JCya-Eea1em1flox/Cya
Common Name:
Eea1-flox
Product ID:
S-CKO-18384
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Eea1-flox
Strain ID
CKOCMP-216238-Eea1-B6J-VB
Gene Name
Product ID
S-CKO-18384
Gene Alias
A430109M19Rik; B230358H09Rik; ZFYVE2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Eea1em1flox/Cya mice (Catalog S-CKO-18384) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000053484
NCBI RefSeq
NM_001001932
Target Region
Exon 6
Size of Effective Region
~0.5 kb
Detailed Document
Overview of Gene Research
Eea1, short for Early Endosomal Antigen 1, is a key protein in endosomal trafficking [2]. It plays a vital role in the fusion of early endosomes, which is an essential step in the endocytic pathway [3,4,5]. Eea1 binds to endosomal membranes containing phosphatidylinositol-3-phosphate (PI(3)P) through its C-terminal FYVE domain, and its association with the endosomal membrane also requires Rab5-GTP and PI(3)K activity [2,3].
In Rett syndrome, a neurodevelopmental disorder caused by loss-of-function mutations in MECP2, Mecp2-deficient neurons lack homeostatic synaptic plasticity likely due to reduced levels of Eea1. Expressing Eea1 in these neurons restores homeostatic synaptic plasticity, suggesting Eea1 as a potential target for intervention in Rett syndrome [1].
In conclusion, Eea1 is crucial for endosomal fusion and trafficking. Its role in maintaining homeostatic synaptic plasticity, as revealed by studies in Mecp2-deficient neurons (a model for Rett syndrome), provides new insights into the disease mechanism and potential treatment strategies. The study of Eea1 using such in vivo models helps to understand its biological functions and its implications in neurological disorders.
References：
1. Xu, Xin, Pozzo-Miller, Lucas. 2017. EEA1 restores homeostatic synaptic plasticity in hippocampal neurons from Rett syndrome mice. In The Journal of physiology, 595, 5699-5712. doi:10.1113/JP274450. https://pubmed.ncbi.nlm.nih.gov/28621434/
2. Larsen, Andreas Haahr, Tata, Lilya, John, Laura H, Sansom, Mark S P. 2021. Membrane-binding mechanism of the EEA1 FYVE domain revealed by multi-scale molecular dynamics simulations. In PLoS computational biology, 17, e1008807. doi:10.1371/journal.pcbi.1008807. https://pubmed.ncbi.nlm.nih.gov/34555023/
3. Simonsen, A, Lippé, R, Christoforidis, S, Zerial, M, Stenmark, H. . EEA1 links PI(3)K function to Rab5 regulation of endosome fusion. In Nature, 394, 494-8. doi:. https://pubmed.ncbi.nlm.nih.gov/9697774/
4. Ramanathan, Harish N, Zhang, Guofeng, Ye, Yihong. 2013. Monoubiquitination of EEA1 regulates endosome fusion and trafficking. In Cell & bioscience, 3, 24. doi:10.1186/2045-3701-3-24. https://pubmed.ncbi.nlm.nih.gov/23701900/
5. Mills, I G, Urbé, S, Clague, M J. . Relationships between EEA1 binding partners and their role in endosome fusion. In Journal of cell science, 114, 1959-65. doi:. https://pubmed.ncbi.nlm.nih.gov/11329382/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen