C57BL/6JCya-Abcc3em1flox/Cya
Common Name:
Abcc3-flox
Product ID:
S-CKO-18385
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Abcc3-flox
Strain ID
CKOCMP-76408-Abcc3-B6J-VB
Gene Name
Product ID
S-CKO-18385
Gene Alias
1700019L09Rik; ABC31; MLP2; MOAT-D; MRP3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Abcc3em1flox/Cya mice (Catalog S-CKO-18385) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000178136
NCBI RefSeq
NM_001363187
Target Region
Exon 9~11
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
ABCC3, also known as MRP3, is a member of the ABCC subfamily within the ATP-Binding Cassette (ABC) transporter family. ABC transporters play a crucial role in the transport of endo-and xenobiotics. ABCC3 is involved in the transport of various substances, including bile acids, and has been associated with multiple biological processes and disease-related pathways [3,4].
In hepatocellular carcinoma (HCC), BRG1 activates ABCC3 transcription, promoting HCC carcinogenesis. ABCC3-silencing in BRG1-overexpressing cells abrogates proliferation and migration, while ABCC3 over-expression rescues these phenotypes in BRG1-depleted cells. High BRG1/ABCC3 expression predicts poor prognosis in HCC patients [1].
In pancreatic ductal adenocarcinoma (PDAC), ABCC3 is overexpressed. Disrupting its function via genetic knockdown reduces pancreatic cancer cell growth in vitro and in vivo, by inhibiting STAT3 and HIF1α signalling pathways [5].
In glioblastoma, ABCC3 expression is associated with poor survival and impaired response to temozolomide, and its high expression is restricted to glioblastoma, making it a suitable target for immunotargeted applications [2].
In colorectal cancer, down-regulation of ABCC3 activates MAPK signalling through the accumulation of deoxycholic acid [4].
In conclusion, ABCC3 is an important transporter involved in multiple disease-related processes. Studies using gene-knockout or knockdown models in various cancer types, such as HCC, PDAC, glioblastoma, and colorectal cancer, have revealed its role in cancer cell proliferation, migration, and response to treatment. These findings suggest that targeting ABCC3 could potentially be a strategy for improving cancer treatment.
References:
1. Liu, Huimin, Yue, Linbo, Hong, Wenxuan, Zhou, Junjing. 2024. SMARCA4 (BRG1) activates ABCC3 transcription to promote hepatocellular carcinogenesis. In Life sciences, 347, 122605. doi:10.1016/j.lfs.2024.122605. https://pubmed.ncbi.nlm.nih.gov/38642845/
2. Ruiz-López, Eduardo, Jovčevska, Ivana, González-Gómez, Ruth, Muyldermans, Serge, Schuhmacher, Alberto J. 2022. Nanobodies targeting ABCC3 for immunotargeted applications in glioblastoma. In Scientific reports, 12, 22581. doi:10.1038/s41598-022-27161-3. https://pubmed.ncbi.nlm.nih.gov/36585418/
3. Ghanem, Carolina I, Manautou, Jose E. . Modulation of Hepatic MRP3/ABCC3 by Xenobiotics and Pathophysiological Conditions: Role in Drug Pharmacokinetics. In Current medicinal chemistry, 26, 1185-1223. doi:10.2174/0929867325666180221142315. https://pubmed.ncbi.nlm.nih.gov/29473496/
4. Sato, Yukihiro, Kobayashi, Minoru, Ohira, Masahiro, Unno, Michiaki, Nakayama, Keiko. 2024. Downregulation of ABCC3 activates MAPK signaling through accumulation of deoxycholic acid in colorectal cancer cells. In Cancer science, 115, 1778-1790. doi:10.1111/cas.16132. https://pubmed.ncbi.nlm.nih.gov/38566304/
5. Adamska, Aleksandra, Ferro, Riccardo, Lattanzio, Rossano, Sala, Gianluca, Falasca, Marco. 2019. ABCC3 is a novel target for the treatment of pancreatic cancer. In Advances in biological regulation, 73, 100634. doi:10.1016/j.jbior.2019.04.004. https://pubmed.ncbi.nlm.nih.gov/31053501/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen