C57BL/6JCya-Cdc23em1flox/Cya
Common Name:
Cdc23-flox
Product ID:
S-CKO-18391
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cdc23-flox
Strain ID
CKOCMP-52563-Cdc23-B6J-VB
Gene Name
Product ID
S-CKO-18391
Gene Alias
6030435O18; D18Ertd243e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cdc23em1flox/Cya mice (Catalog S-CKO-18391) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000133181
NCBI RefSeq
NM_178347
Target Region
Exon 7~9
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Cdc23, also related to Mcm10 in some contexts, is a core subunit of the anaphase-promoting complex or cyclosome (APC/C) and is involved in regulating mitosis in eukaryotes [2,4]. It is associated with pathways like the cell cycle, and its abnormal regulation can impact various biological processes and disease states. Genetic models, such as in yeast and mice, have been crucial for studying its functions.
In cancer research, knockdown of Cdc23 in liver cancer cell lines and in xenograft models inhibited cell proliferation, migration, and invasion via regulating the epithelial-mesenchymal transition (EMT) process, suggesting it could be a therapeutic target for liver cancer [1]. In papillary thyroid cancer, functional knockdown of Cdc23 led to cell cycle arrest in S and G(2)M phases, inhibiting cellular proliferation, tumor spheroid formation, and anchorage-independent growth, indicating its role in thyroid cancer initiation and progression [3]. In female infertility, homozygous missense variants in Cdc23 in three infertile individuals caused oocyte maturation defects, and Cdc23Y329C/Y329C mice mimicked the patients' phenotype, revealing the gene's importance in oocyte maturation [2]. In porcine skeletal muscle development, in vitro cell experiments showed that Cdc23 promoted myoblast proliferation and positively regulated skeletal muscle satellite cell differentiation [4].
In conclusion, Cdc23 is essential for processes like cell cycle regulation, oocyte maturation, and muscle development. Gene-knockdown and mouse models have revealed its significance in cancer development, such as in liver and thyroid cancer, and in female infertility, providing insights into potential therapeutic targets and genetic markers for these disease areas.
References:
1. Zhang, Yang, Luo, Lianghua, Fu, Chengchao, Li, Yong, Xiong, Jianbo. 2023. CDC23 knockdown suppresses the proliferation, migration and invasion of liver cancer via the EMT process. In Oncology letters, 26, 291. doi:10.3892/ol.2023.13877. https://pubmed.ncbi.nlm.nih.gov/37274472/
2. Fan, Huizhen, Zhou, Zhou, Zheng, Wei, Sang, Qing, Wang, Lei. 2023. Homozygous variants in CDC23 cause female infertility characterized by oocyte maturation defects. In Human genetics, 142, 1621-1631. doi:10.1007/s00439-023-02606-5. https://pubmed.ncbi.nlm.nih.gov/37768355/
3. Zhang, Lisa, Rahbari, Reza, He, Mei, Kebebew, Electron. 2011. CDC23 regulates cancer cell phenotype and is overexpressed in papillary thyroid cancer. In Endocrine-related cancer, 18, 731-42. doi:10.1530/ERC-11-0181. https://pubmed.ncbi.nlm.nih.gov/21990323/
4. Xie, Su, Liu, Quan, Fu, Chong, Han, Min, Li, Changchun. 2024. Molecular Regulation of Porcine Skeletal Muscle Development: Insights from Research on CDC23 Expression and Function. In International journal of molecular sciences, 25, . doi:10.3390/ijms25073664. https://pubmed.ncbi.nlm.nih.gov/38612477/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen