C57BL/6JCya-Asap1em1flox/Cya
Common Name:
Asap1-flox
Product ID:
S-CKO-18395
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Asap1-flox
Strain ID
CKOCMP-13196-Asap1-B6J-VB
Gene Name
Product ID
S-CKO-18395
Gene Alias
DEF-1; Ddef1; PAP; mKIAA1249; s19
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Asap1em1flox/Cya mice (Catalog S-CKO-18395) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000177374
NCBI RefSeq
NM_010026
Target Region
Exon 4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
ASAP1, an ADP-ribosylation factor GTPase-activating protein, has multiple functions including regulating cytoskeletal dynamics, small GTP-binding protein receptor recycling, and intracellular vesicle trafficking [4,5,6]. It is involved in pathways such as the IQGAP1/CDC42 pathway [1], NRAS/MEK1/ERK1-2 signaling [2], and the Wnt/β-catenin pathway [7]. These functions are crucial for various biological processes, and its dysregulation is associated with cancer and infectious diseases.
In cancer, ASAP1 shows diverse effects. In gastric cancer, its overexpression activates the IQGAP1/CDC42 pathway, promoting tumor progression and chemotherapy resistance [1]. In glioblastoma, circular RNA ASAP1 promotes tumorigenesis and temozolomide resistance via the NRAS/MEK1/ERK1-2 signaling [2]. In hepatocellular carcinoma, high ASAP1 expression is associated with aggressive clinicopathological characteristics and poor outcomes [3]. However, in the MMTV-PyMT model of luminal breast cancer, ablation of ASAP1 accelerates tumorigenesis and promotes metastasis by activating AKT [5]. In zebrafish, knockdown of asap1 homologs increases susceptibility to Mycobacterium marinum infection by reducing macrophage migration [4].
In conclusion, ASAP1 is a key regulator in multiple biological processes. Its role in cancer and infectious diseases has been revealed through various in vivo models. These findings contribute to understanding disease mechanisms, with implications for potential therapeutic strategies targeting ASAP1-related pathways in these disease areas.
References：
1. Xie, Wangkai, Han, Zheng, Zuo, Ziyi, Xue, Xiangyang, Lin, Kezhi. 2023. ASAP1 activates the IQGAP1/CDC42 pathway to promote tumor progression and chemotherapy resistance in gastric cancer. In Cell death & disease, 14, 124. doi:10.1038/s41419-023-05648-9. https://pubmed.ncbi.nlm.nih.gov/36792578/
2. Wei, Yutian, Lu, Chenfei, Zhou, Peng, Shi, ZhuMei, You, Yongping. . EIF4A3-induced circular RNA ASAP1 promotes tumorigenesis and temozolomide resistance of glioblastoma via NRAS/MEK1/ERK1-2 signaling. In Neuro-oncology, 23, 611-624. doi:10.1093/neuonc/noaa214. https://pubmed.ncbi.nlm.nih.gov/32926734/
3. Bang, Seongsik, Jee, Seungyun, Son, Hwangkyu, Kim, Hyunsung, Paik, Seungsam. 2022. Clinicopathological Implications of ASAP1 Expression in Hepatocellular Carcinoma. In Pathology oncology research : POR, 28, 1610635. doi:10.3389/pore.2022.1610635. https://pubmed.ncbi.nlm.nih.gov/36110251/
4. Cui, Jia, Chen, Guangxin, Wen, Da, Zhao, Zhonghua, Wu, Changxin. 2020. Asap1 Affects the Susceptibility of Zebrafish to Mycobacterium by Regulating Macrophage Migration. In Frontiers in cellular and infection microbiology, 10, 519503. doi:10.3389/fcimb.2020.519503. https://pubmed.ncbi.nlm.nih.gov/33194781/
5. Schreiber, Caroline, Gruber, Annette, Roßwag, Sven, Garvalov, Boyan K, Sleeman, Jonathan P. 2022. Loss of ASAP1 in the MMTV-PyMT model of luminal breast cancer activates AKT, accelerates tumorigenesis, and promotes metastasis. In Cancer letters, 533, 215600. doi:10.1016/j.canlet.2022.215600. https://pubmed.ncbi.nlm.nih.gov/35181478/
6. Gasilina, Anjelika, Vitali, Teresa, Luo, Ruibai, Jian, Xiaoying, Randazzo, Paul A. 2019. The ArfGAP ASAP1 Controls Actin Stress Fiber Organization via Its N-BAR Domain. In iScience, 22, 166-180. doi:10.1016/j.isci.2019.11.015. https://pubmed.ncbi.nlm.nih.gov/31785555/
7. He, Jiaqi, Liu, Han, Cai, Jianhua, Wang, Jiwen, Liu, Houbao. 2024. ASAP1 promotes extrahepatic cholangiocarcinoma progression by regulating the Wnt/β-catenin pathway in vitro and in vivo. In American journal of cancer research, 14, 5178-5192. doi:10.62347/RKQX3504. https://pubmed.ncbi.nlm.nih.gov/39659924/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen