C57BL/6JCya-Faf1em1flox/Cya
Common Name:
Faf1-flox
Product ID:
S-CKO-18414
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Faf1-flox
Strain ID
CKOCMP-14084-Faf1-B6J-VB
Gene Name
Product ID
S-CKO-18414
Gene Alias
Dffrx; Fam
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Faf1em1flox/Cya mice (Catalog S-CKO-18414) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102724
NCBI RefSeq
NM_007983
Target Region
Exon 3
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Faf1, or Fas-associated factor 1, is an evolutionarily conserved protein with multiple protein-interaction domains. Initially recognized as part of the FAS death-inducing signaling complex, it is involved in diverse biological processes. Faf1 has been shown to be important in normal development, neuronal cell survival, and is also associated with pathways like apoptosis, NFκB activity, ubiquitination, and proteasomal degradation [2].
In terms of disease-related findings, in KO mice, Faf1 deficiency did not lead to pituitary lesions or differences in corticosterone secretion, despite the identification of potentially pathogenic Faf1 variants in Cushing's disease patients [1]. Faf1 knockout mice were more resistant to RNA virus infection, with Faf1 deficiency in myeloid cells enhancing innate signaling and reducing viral load and morbidity [3]. In a Parkinson's disease mouse model, targeting Faf1 with KM-819 restored autophagic flux for α-synuclein degradation [4]. In breast cancer, Faf1-knockout animals and various models revealed that Faf1 phosphorylation by AKT accumulates TGF-β type II receptor and drives breast cancer metastasis [5].
In conclusion, Faf1 plays crucial roles in multiple biological processes and disease conditions. Gene knockout mouse models have been instrumental in uncovering its functions in antiviral immunity, neurodegenerative diseases like Parkinson's, and cancers such as breast cancer and potentially pituitary tumors. Understanding Faf1's functions provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Nguyen, Matthew, Maria, Andrea Gutierrez, Faucz, Fabio R, Stratakis, Constantine A, Tatsi, Christina. 2023. FAF1 Gene Involvement in Pituitary Corticotroph Tumors. In Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme, 56, 604-610. doi:10.1055/a-2192-1761. https://pubmed.ncbi.nlm.nih.gov/38065537/
2. Menges, Craig W, Altomare, Deborah A, Testa, Joseph R. 2009. FAS-associated factor 1 (FAF1): diverse functions and implications for oncogenesis. In Cell cycle (Georgetown, Tex.), 8, 2528-34. doi:. https://pubmed.ncbi.nlm.nih.gov/19597341/
3. Dai, Tong, Wu, Liming, Wang, Shuai, Huang, Huizhe, Zhang, Long. 2018. FAF1 Regulates Antiviral Immunity by Inhibiting MAVS but Is Antagonized by Phosphorylation upon Viral Infection. In Cell host & microbe, 24, 776-790.e5. doi:10.1016/j.chom.2018.10.006. https://pubmed.ncbi.nlm.nih.gov/30472208/
4. Kim, Bok-Seok, Song, Jin-A, Jang, Ki-Hong, Lee, Jae Moon, Kim, Eunhee. 2022. Pharmacological Intervention Targeting FAF1 Restores Autophagic Flux for α-Synuclein Degradation in the Brain of a Parkinson's Disease Mouse Model. In ACS chemical neuroscience, 13, 806-817. doi:10.1021/acschemneuro.1c00828. https://pubmed.ncbi.nlm.nih.gov/35230076/
5. Xie, Feng, Jin, Ke, Shao, Li, Zhou, Fangfang, Zhang, Long. 2017. FAF1 phosphorylation by AKT accumulates TGF-β type II receptor and drives breast cancer metastasis. In Nature communications, 8, 15021. doi:10.1038/ncomms15021. https://pubmed.ncbi.nlm.nih.gov/28443643/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen