C57BL/6JCya-Bicraem1flox/Cya
Common Name:
Bicra-flox
Product ID:
S-CKO-18431
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Bicra-flox
Strain ID
CKOCMP-243842-Bicra-B6J-VB
Gene Name
Product ID
S-CKO-18431
Gene Alias
Gltscr1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bicraem1flox/Cya mice (Catalog S-CKO-18431) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000094821
NCBI RefSeq
NM_001081418
Target Region
Exon 7~10
Size of Effective Region
~3.6 kb
Detailed Document
Overview of Gene Research
Bicra, also known as GLTSCR1, encodes a subunit of the non-canonical BAF (ncBAF) chromatin remodeling complex. As a member of the SWI/SNF family, it is involved in chromatin remodeling, which is crucial for regulating gene expression, and thus has overall biological importance in various biological processes [1].
In zebrafish, a mutation of bicra mimicking a loss-of-function variant leads to craniofacial defects, similar to the dysmorphic facial features seen in humans with pathogenic BICRA variants [1]. In Drosophila, loss of Bicra impairs learning and choice abilities, specifically in male courtship learning and choice performance, indicating its role in cognition-related behaviors [2]. In mice, homozygous loss of Bicra using Nuclease technology results in blood-related defects, including defective erythroblastic islands and irregularly sized red blood cells due to a defect in fetal liver macrophages responsible for erythrocyte maturation [5].
In conclusion, Bicra plays essential roles in multiple biological processes, as revealed through studies in zebrafish, Drosophila, and mouse models. These model-based research findings show its significance in craniofacial development, cognitive behaviors, and erythrocyte maturation. The loss-of-function studies in these models contribute to understanding the role of Bicra in related human diseases, such as Coffin-Siris syndrome 12, which is associated with diverse neurodevelopmental delays [1,3,4].
References:
1. Barish, Scott, Barakat, Tahsin Stefan, Michel, Brittany C, Scott, Daryl A, Bellen, Hugo J. 2020. BICRA, a SWI/SNF Complex Member, Is Associated with BAF-Disorder Related Phenotypes in Humans and Model Organisms. In American journal of human genetics, 107, 1096-1112. doi:10.1016/j.ajhg.2020.11.003. https://pubmed.ncbi.nlm.nih.gov/33232675/
2. Sun, Ying, Li, Zhuojie, Li, Wenzhe, Xue, Lei. 2021. Loss of Bicra impairs Drosophila learning and choice abilities. In Neuroscience letters, 769, 136432. doi:10.1016/j.neulet.2021.136432. https://pubmed.ncbi.nlm.nih.gov/34974109/
3. Tu, Youquan, Fang, Chunyan, Xu, Jian, Liang, Mengmeng, Yang, Zuozhen. 2023. A de novo variant of BICRA results in Coffin-Siris syndrome 12. In Molecular genetics & genomic medicine, 11, e2250. doi:10.1002/mgg3.2250. https://pubmed.ncbi.nlm.nih.gov/37485815/
4. Asadauskaitė, Greta, Morkūnienė, Aušra, Utkus, Algirdas, Burnytė, Birutė. 2022. Identification of a novel BICRA variant leading to the newly described Coffin-Siris syndrome 12. In Brain & development, 45, 185-190. doi:10.1016/j.braindev.2022.11.003. https://pubmed.ncbi.nlm.nih.gov/36437209/
5. Sood, Surbhi, Alpsoy, Aktan, Jiao, Guanming, Hutchcroft, Jill, Dykhuizen, Emily. 2024. Loss of Bicra/Gltscr1 leads to a defect in fetal liver macrophages responsible for erythrocyte maturation in mice. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.10.17.618940. https://pubmed.ncbi.nlm.nih.gov/39464106/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen