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C57BL/6JCya-Chek1em1flox/Cya
Common Name:
Chek1-flox
Product ID:
S-CKO-18448
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Chek1-flox
Strain ID
CKOCMP-12649-Chek1-B6J-VB
Gene Name
Chek1
Product ID
S-CKO-18448
Gene Alias
Chk1; rad27
Background
C57BL/6JCya
NCBI ID
12649
Modification
Conditional knockout
Chromosome
9
Phenotype
MGI:1202065
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Chek1em1flox/Cya mice (Catalog S-CKO-18448) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034625
NCBI RefSeq
NM_007691
Target Region
Exon 3~5
Size of Effective Region
~3.8 kb
Detailed Document
Click here to download >>
Overview of Gene Research
CHEK1, also known as checkpoint kinase 1, is a key signal transducer inside the genomic integrity checkpoints. It is involved in the cell cycle checkpoint pathways, responding to DNA damage. When DNA is under stress from endogenous or exogenous damaging agents, the cell activates the ATM-Chk2 and ATR-Chk1 pathways, with CHEK1 being a downstream effector in the ATR-Chk1 pathway. This pathway is crucial for the cell to either halt the cell cycle or initiate apoptosis in response to DNA damage, ensuring genome stability [4,5].

CHEK1 is overexpressed in a range of cancers such as lung adenocarcinoma, multiple myeloma, and is associated with TP53 mutations in lung tumors. In lung adenocarcinoma, its overexpression, potentially triggered by promoter methylation, amplification, and miRNA regulation, signals a poor prognosis. In multiple myeloma, high CHEK1 expression is linked to poor outcomes, and it promotes cellular proliferation, drug-resistance, chromosomal instability, and osteoclast differentiation. In lung squamous carcinoma, miR-139-3p can target CHEK1, modulating DNA repair and cell viability. Also, in diabetic nephropathy, CHEK1 may be a biomarker of glomerular epithelial cell injury [1,2,3,6,7,8].

In conclusion, CHEK1 is essential for maintaining genomic integrity through its role in the DNA damage response and cell cycle regulation. Its dysregulation is associated with various cancers and other diseases, highlighting its potential as a biomarker and therapeutic target. Studies on CHEK1 in different disease models have provided insights into its functions in disease development, which could be valuable for future treatment strategies.

References:
1. Tan, Zhibo, Chen, Min, Wang, Ying, Li, Ying, Liu, Yajie. 2021. CHEK1: a hub gene related to poor prognosis for lung adenocarcinoma. In Biomarkers in medicine, 16, 83-100. doi:10.2217/bmm-2021-0919. https://pubmed.ncbi.nlm.nih.gov/34882011/
2. Liu, Xiao-Ping, Huang, Qiao, Yin, Xiao-Hong, Yan, Xin-Hui, He, Li. . Strong Correlation between the Expression of CHEK1 and Clinicopathological Features of Patients with Multiple Myeloma. In Critical reviews in eukaryotic gene expression, 30, 349-357. doi:10.1615/CritRevEukaryotGeneExpr.2020027084. https://pubmed.ncbi.nlm.nih.gov/32894664/
3. Gu, Chunyan, Wang, Wang, Tang, Xiaozhu, Pan, Jingxuan, Yang, Ye. 2021. CHEK1 and circCHEK1_246aa evoke chromosomal instability and induce bone lesion formation in multiple myeloma. In Molecular cancer, 20, 84. doi:10.1186/s12943-021-01380-0. https://pubmed.ncbi.nlm.nih.gov/34090465/
4. Levy, Antonin, Albiges-Sauvin, Laurence, Massard, Christophe, Soria, Jean-Charles, Deutsch, Eric. . [Cell cycle, mitosis and therapeutic applications]. In Bulletin du cancer, 98, 1037-45. doi:. https://pubmed.ncbi.nlm.nih.gov/21669563/
5. Smith, Joanne, Tho, Lye Mun, Xu, Naihan, Gillespie, David A. . The ATM-Chk2 and ATR-Chk1 pathways in DNA damage signaling and cancer. In Advances in cancer research, 108, 73-112. doi:10.1016/B978-0-12-380888-2.00003-0. https://pubmed.ncbi.nlm.nih.gov/21034966/
6. Õsz, Ágnes, Aszódi, Boglárka, Vajda, Réka, Casanova, Emilio, Gyõrffy, Balázs. 2019. [CHEK1 expression and inhibitors in TP53 mutant cancer]. In Magyar onkologia, 63, 345-352. doi:. https://pubmed.ncbi.nlm.nih.gov/31821389/
7. Wang, Dan, Du, Yuejuan, Huang, Ran, Liu, Fang, Wang, Pu. 2023. Bioinformatic analysis of CHEK1 as a marker of glomerular epithelial cell injury in diabetic nephropathy. In Cellular and molecular biology (Noisy-le-Grand, France), 69, 209-213. doi:10.14715/cmb/2023.69.8.32. https://pubmed.ncbi.nlm.nih.gov/37715382/
8. Zheng, Xiaoyu, Zhang, Yingchun, Wu, Shaojun, Jiang, Bin, Liu, Yongchun. 2022. MiR-139-3p Targets CHEK1 Modulating DNA Repair and Cell Viability in Lung Squamous Carcinoma Cells. In Molecular biotechnology, 64, 832-840. doi:10.1007/s12033-022-00462-8. https://pubmed.ncbi.nlm.nih.gov/35150405/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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