C57BL/6JCya-Atp2a2em1flox/Cya
Common Name:
Atp2a2-flox
Product ID:
S-CKO-18456
Background:
C57BL/6JCya
Product Type
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Basic Information
Strain Name
Atp2a2-flox
Strain ID
CKOCMP-11938-Atp2a2-B6J-VB
Gene Name
Product ID
S-CKO-18456
Gene Alias
9530097L16Rik; D5Wsu150e; SERCA2; SERCA2B; Serca2a; mKIAA4195
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Atp2a2em1flox/Cya mice (Catalog S-CKO-18456) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031423
NCBI RefSeq
NM_001110140
Target Region
Exon 7~8
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Atp2a2, also known as SERCA2, encodes a Ca2+ pump on the endoplasmic reticulum (ER) membrane. It is crucial for intracellular Ca2+ signaling, which is involved in multiple biological processes [1,2,3,4].
In Atp2a2 heterozygous brain-specific conditional knockout (hetero cKO) mice, the ER membranes from the brain showed decreased Ca2+ uptake activity. The hetero cKO mice had slower decays of cytosolic Ca2+ levels in neurons after depolarization, leading to altered behavioral responses and fear memory impairments, along with enhanced dopamine signaling and higher extracellular dopamine levels in the NAc. This indicates that haploinsufficiency of Atp2a2 in the brain causes prolonged cytosolic Ca2+ transients and potentially enhanced dopamine signaling, a feature associated with mood disorders and schizophrenia [1].
In conclusion, Atp2a2 is essential for maintaining proper intracellular Ca2+ homeostasis through its role as an ER Ca2+ pump. The study of Atp2a2 using gene knockout models, such as the brain-specific hetero cKO mice, has revealed its significance in neuropsychiatric disorders, highlighting the potential link between its mutations and the development of these conditions.
References：
1. Nakajima, Kazuo, Ishiwata, Mizuho, Weitemier, Adam Z, McHugh, Thomas J, Kato, Tadafumi. . Brain-specific heterozygous loss-of-function of ATP2A2, endoplasmic reticulum Ca2+ pump responsible for Darier's disease, causes behavioral abnormalities and a hyper-dopaminergic state. In Human molecular genetics, 30, 1762-1772. doi:10.1093/hmg/ddab137. https://pubmed.ncbi.nlm.nih.gov/34104969/
2. Kiener, Sarah, Yang, Ching, Rich, Naomi, Mauldin, Elizabeth A, Leeb, Tosso. 2023. Heterozygous ATP2A2 missense variant identified in a Shih Tzu with Darier disease. In Animal genetics, 54, 558-561. doi:10.1111/age.13314. https://pubmed.ncbi.nlm.nih.gov/36883421/
3. Nellen, Ruud G L, Steijlen, Peter M, van Steensel, Maurice A M, Frank, Jorge, van Geel, Michel. 2017. Mendelian Disorders of Cornification Caused by Defects in Intracellular Calcium Pumps: Mutation Update and Database for Variants in ATP2A2 and ATP2C1 Associated with Darier Disease and Hailey-Hailey Disease. In Human mutation, 38, 343-356. doi:10.1002/humu.23164. https://pubmed.ncbi.nlm.nih.gov/28035777/
4. Li, Min, Higashi, Naoyuki, Nakano, Hajime, Saeki, Hidehisa. . An ATP2A2 Missense Mutation in a Japanese Family with Darier Disease: A Case Report and Review of the Japanese Darier Disease Patients with ATP2A2 Mutations. In Journal of Nippon Medical School = Nippon Ika Daigaku zasshi, 84, 246-250. doi:10.1272/jnms.84.246. https://pubmed.ncbi.nlm.nih.gov/29142187/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen