C57BL/6JCya-Qprtem1flox/Cya
Common Name:
Qprt-flox
Product ID:
S-CKO-18464
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Qprt-flox
Strain ID
CKOCMP-67375-Qprt-B6J-VB
Gene Name
Product ID
S-CKO-18464
Gene Alias
2410027J01Rik; QPRTase
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Qprtem1flox/Cya mice (Catalog S-CKO-18464) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032912
NCBI RefSeq
NM_133686
Target Region
Exon 2~4
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Qprt, short for Quinolinate phosphoribosyltransferase, is a rate-limiting enzyme encoding the uronic acid pathway. It is involved in cell cycle progression, cancer cell metabolism, and is a key enzyme in the de novo biosynthesis of nicotinamide adenine dinucleotide (NAD+) from tryptophan [2,3,4,7].
In QPRT-KO mice, the conversion ratio of tryptophan to quinolinic acid was higher than expected, but QPRT was not the rate-limiting enzyme in the conversion of tryptophan to nicotinamide (Nam) [4]. Deletion of Qprt in mice led to decreased ovarian NAD+ levels, accelerating ovarian aging, with subfertility, irregular estrous cycles, and reduced ovarian reserve [6]. In the context of acute kidney injury (AKI), QPRT expression declined in two models of AKI. Haploinsufficient (QPRT+/-) mice had higher quinolinate, lower NAD+, and higher AKI susceptibility [1,7]. In breast cancer, high QPRT expression was associated with a poor prognosis and promoted cancer progression through the PI3K/Akt signaling pathway [2,3,5]. Knockdown of QPRT in human embryonic kidney cells affected cell-cycle progression and proliferation [8].
In conclusion, Qprt is crucial for NAD+ biosynthesis and plays important roles in multiple biological processes and disease conditions. The use of Qprt KO/CKO mouse models has revealed its significance in ovarian aging, AKI, and cancer, helping to understand the underlying mechanisms and potentially providing new targets for treatment.
References:
1. Clark, Amanda J, Saade, Marie Christelle, Vemireddy, Vamsidhara, Zsengellér, Zsuzsanna K, Parikh, Samir M. 2023. Hepatocyte nuclear factor 4α mediated quinolinate phosphoribosylltransferase (QPRT) expression in the kidney facilitates resilience against acute kidney injury. In Kidney international, 104, 1150-1163. doi:10.1016/j.kint.2023.09.013. https://pubmed.ncbi.nlm.nih.gov/37783445/
2. Zhou, Lili, Mu, Lin, Jiang, Wenyan, Yang, Qi. 2022. QPRT Acts as an Independent Prognostic Factor in Invasive Breast Cancer. In Journal of oncology, 2022, 6548644. doi:10.1155/2022/6548644. https://pubmed.ncbi.nlm.nih.gov/35251175/
3. Yan, Yiqing, Li, Lun, Wang, Zixin, Xia, Zhenkun, Yi, Wenjun. 2023. A comprehensive analysis of the role of QPRT in breast cancer. In Scientific reports, 13, 15414. doi:10.1038/s41598-023-42566-4. https://pubmed.ncbi.nlm.nih.gov/37723185/
4. Shibata, Katsumi, Fukuwatari, Tsutomu. 2016. Organ Correlation with Tryptophan Metabolism Obtained by Analyses of TDO-KO and QPRT-KO Mice. In International journal of tryptophan research : IJTR, 9, 1-7. doi:10.4137/IJTR.S37984. https://pubmed.ncbi.nlm.nih.gov/27147825/
5. Zhang, Qun, Ding, Xinwang, Lu, Hongzhao. 2022. Increased Expression of QPRT in Breast Cancer Infers a Poor Prognosis and Is Correlated to Immunocytes Infiltration. In Journal of healthcare engineering, 2022, 6482878. doi:10.1155/2022/6482878. https://pubmed.ncbi.nlm.nih.gov/35345651/
6. Yang, Qingling, Li, Hui, Wang, Huan, Xu, Jianmin, Sun, Yingpu. 2023. Deletion of enzymes for de novo NAD+ biosynthesis accelerated ovarian aging. In Aging cell, 22, e13904. doi:10.1111/acel.13904. https://pubmed.ncbi.nlm.nih.gov/37332134/
7. Poyan Mehr, Ali, Tran, Mei T, Ralto, Kenneth M, Berg, Anders H, Parikh, Samir M. 2018. De novo NAD+ biosynthetic impairment in acute kidney injury in humans. In Nature medicine, 24, 1351-1359. doi:10.1038/s41591-018-0138-z. https://pubmed.ncbi.nlm.nih.gov/30127395/
8. Zhou, Xiao Y, Zheng, Hao Y, Han, Li, Liu, Guan N, Ding, Lian S. 2021. Copy Number Variations Analysis Identifies QPRT as a Candidate Gene Associated With Susceptibility for Solitary Functioning Kidney. In Frontiers in genetics, 12, 575830. doi:10.3389/fgene.2021.575830. https://pubmed.ncbi.nlm.nih.gov/34079576/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen