C57BL/6JCya-Zmynd8em1flox/Cya
Common Name:
Zmynd8-flox
Product ID:
S-CKO-18469
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Zmynd8-flox
Strain ID
CKOCMP-228880-Zmynd8-B6J-VB
Gene Name
Product ID
S-CKO-18469
Gene Alias
1110013E22Rik; 2010005I16Rik; 3632413B07Rik; Prkcbp1; RACK7
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zmynd8em1flox/Cya mice (Catalog S-CKO-18469) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000109269
NCBI RefSeq
NM_001291158
Target Region
Exon 4
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Zmynd8, also known as Zinc finger MYND-type containing 8, is a transcription factor, histone H3-interacting protein, and putative chromatin reader/effector. It plays a crucial role in regulating transcription during normal cellular growth and is involved in multiple biological processes and pathways [2].
In breast cancer, Zmynd8 upregulation in breast cancer stem cells (BCSCs) reduces ROS and iron to inhibit ferroptosis, leading to BCSC expansion and tumor initiation. It does this through a two-fold post-translational regulation of NRF2 [1].
In acute myeloid leukemia (AML), Zmynd8 directly activates IRF8 in parallel with the MYC proto-oncogene, and is essential for AML proliferation in vitro and in vivo [3].
In intestinal cancer, YAP-mediated Zmynd8 expression sensitizes tumors to the inhibition of the mevalonate (MVA) pathway, and disruption of the Zmynd8-dependent MVA pathway restricts the self-renewal capacity of Lgr5+ intestinal stem cells and intestinal tumorigenesis [4].
In conclusion, Zmynd8 is involved in various biological processes and disease conditions. Its role in cancer, such as in breast, AML, and intestinal cancers, has been revealed through functional studies including in vivo models. These findings suggest that Zmynd8 could potentially be a therapeutic target, and its study helps in understanding the molecular mechanisms underlying cancer development [1,2,3,4].
References：
1. Luo, Maowu, Bao, Lei, Xue, Yuanyuan, Wang, Yingfei, Luo, Weibo. 2024. ZMYND8 protects breast cancer stem cells against oxidative stress and ferroptosis through activation of NRF2. In The Journal of clinical investigation, 134, . doi:10.1172/JCI171166. https://pubmed.ncbi.nlm.nih.gov/38488001/
2. Chen, Yun, Tsai, Ya-Hui, Tseng, Sheng-Hong. 2021. Regulation of ZMYND8 to Treat Cancer. In Molecules (Basel, Switzerland), 26, . doi:10.3390/molecules26041083. https://pubmed.ncbi.nlm.nih.gov/33670804/
3. Cao, Zhendong, Budinich, Krista A, Huang, Hua, Berger, Shelley L, Shi, Junwei. 2021. ZMYND8-regulated IRF8 transcription axis is an acute myeloid leukemia dependency. In Molecular cell, 81, 3604-3622.e10. doi:10.1016/j.molcel.2021.07.018. https://pubmed.ncbi.nlm.nih.gov/34358447/
4. Pan, Qiang, Zhong, Shanshan, Wang, Hanling, Xiao, Yichuan, Qin, Jun. 2021. The ZMYND8-regulated mevalonate pathway endows YAP-high intestinal cancer with metabolic vulnerability. In Molecular cell, 81, 2736-2751.e8. doi:10.1016/j.molcel.2021.04.009. https://pubmed.ncbi.nlm.nih.gov/33932349/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen