C57BL/6JCya-Ptrh1em1flox/Cya
Common Name:
Ptrh1-flox
Product ID:
S-CKO-18479
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ptrh1-flox
Strain ID
CKOCMP-329384-Ptrh1-B6J-VA
Gene Name
Product ID
S-CKO-18479
Gene Alias
2210013M04Rik; PTH
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ptrh1em1flox/Cya mice (Catalog S-CKO-18479) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000066352
NCBI RefSeq
NM_178595
Target Region
Exon 2~5
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Ptrh1, short for peptidyl-tRNA hydrolase 1 homolog, is an RNA binding protein (RBP) with significant functions in multiple biological processes. It is involved in the ribosome-associated quality control (RQC) pathway, where it can cleave non-ubiquitinated NC-tRNA in non-ubiquitinated 60S RNCs and 80S RNCs formed on non-stop mRNAs [2]. In addition, it is associated with pathways related to tumor immunity and cell translation processes, making it biologically important in maintaining normal cellular functions and immune responses [1,2].
In pancreatic cancer, high glucose enhances the stability of the PD-L1 mRNA in pancreatic tumor cells by downregulating PTRH1 through RAS signaling pathway activation following epidermal growth factor receptor (EGFR) stimulation. Overexpression of PTRH1 in pancreatic cells significantly suppresses PD-L1 expression and improves the proportion and cytotoxic function of CD8+ T cells in the pancreatic tumor microenvironment (TME) of diabetic mice, indicating its key role in the regulation of PD-L1 by high glucose and its relation to anti-tumor immunity in the pancreatic TME [1].
In conclusion, Ptrh1 is crucial in the RQC pathway for handling interrupted translation and in regulating PD-L1 expression in the context of pancreatic cancer. The findings from the study on pancreatic cancer using diabetic murine models highlight its significance in tumor-associated immune regulation, suggesting that understanding Ptrh1 function may provide insights into new strategies for treating pancreatic cancer and potentially other related diseases [1,2].
References:
1. Gao, Chenggang, Chen, Jiaoshun, Bai, Jianwei, Shen, Qiang, Yin, Tao. 2023. High glucose-upregulated PD-L1 expression through RAS signaling-driven downregulation of PTRH1 leads to suppression of T cell cytotoxic function in tumor environment. In Journal of translational medicine, 21, 461. doi:10.1186/s12967-023-04302-4. https://pubmed.ncbi.nlm.nih.gov/37434177/
2. Kuroha, Kazushige, Zinoviev, Alexandra, Hellen, Christopher U T, Pestova, Tatyana V. 2018. Release of Ubiquitinated and Non-ubiquitinated Nascent Chains from Stalled Mammalian Ribosomal Complexes by ANKZF1 and Ptrh1. In Molecular cell, 72, 286-302.e8. doi:10.1016/j.molcel.2018.08.022. https://pubmed.ncbi.nlm.nih.gov/30244831/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen