C57BL/6JCya-Tnnt2em1flox/Cya
Common Name:
Tnnt2-flox
Product ID:
S-CKO-18485
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tnnt2-flox
Strain ID
CKOCMP-21956-Tnnt2-B6J-VB
Gene Name
Product ID
S-CKO-18485
Gene Alias
Tnt; cTnT
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tnnt2em1flox/Cya mice (Catalog S-CKO-18485) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000188028
NCBI RefSeq
NM_001130174
Target Region
Exon 6~10
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Tnnt2, encoding cardiac muscle troponin T, is a key player in the calcium regulation of actin thin filament function, essential for cardiac muscle contraction [2]. It is involved in pathways related to cardiomyocyte contraction and has significant biological importance in heart function. Genetic models, such as those using human induced pluripotent stem cell models, are valuable for studying Tnnt2.
Pathogenic Tnnt2 variants can cause hypertrophic and dilated cardiomyopathies. Hypertrophic cardiomyopathy-associated Tnnt2 variants increase cardiac microtissue contraction, while dilated cardiomyopathy-associated variants decrease it, both paralleling changes in myofilament calcium affinity. Tnnt2 variant-dependent changes in sarcomere contractile function induce graded regulation of 101 gene transcripts, including MAPK signaling targets, HOPX, and NPPB. Based on NPPB reporter activity and cardiac microtissue contraction, some Tnnt2 variants' pathogenicity classification could be re-evaluated [1]. In colorectal cancer, Tnnt2 may act as an oncogene, promoting cell proliferation, invasion, and metastasis through the EGFR/HER2/EMT signal axis. Its overexpression is associated with inferior prognosis, and it may be a potential biomarker and therapeutic target [3,4].
In conclusion, Tnnt2 is crucial for cardiac muscle contraction. Model-based research has revealed its role in cardiomyopathies and colorectal cancer. Understanding Tnnt2 through these models helps in improving heart failure risk determination, treatment efficacy, and provides new insights into disease pathogenesis, especially in the context of cardiomyopathies and colorectal cancer.
References:
1. Pettinato, Anthony M, Ladha, Feria A, Mellert, David J, Chen, Yu-Sheng, Hinson, J Travis. 2020. Development of a Cardiac Sarcomere Functional Genomics Platform to Enable Scalable Interrogation of Human TNNT2 Variants. In Circulation, 142, 2262-2275. doi:10.1161/CIRCULATIONAHA.120.047999. https://pubmed.ncbi.nlm.nih.gov/33025817/
2. Wei, Bin, Jin, J-P. 2016. TNNT1, TNNT2, and TNNT3: Isoform genes, regulation, and structure-function relationships. In Gene, 582, 1-13. doi:10.1016/j.gene.2016.01.006. https://pubmed.ncbi.nlm.nih.gov/26774798/
3. Liu, Yifan, Meng, Ze, Niu, Junqiang, Liu, Yibing, Zhou, Zhiguo. 2023. Cardiac tropoini T (TNNT2) plays a potential oncogenic role in colorectal carcinogenesis. In Cancer cell international, 23, 146. doi:10.1186/s12935-023-02977-9. https://pubmed.ncbi.nlm.nih.gov/37481519/
4. Jing, Li, Feng, Li, Zhou, Zhiguo, Ren, Zhixue, Liu, Yibing. 2020. TNNT2 as a potential biomarker for the progression and prognosis of colorectal cancer. In Oncology reports, 44, 628-636. doi:10.3892/or.2020.7637. https://pubmed.ncbi.nlm.nih.gov/32627044/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen