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C57BL/6JCya-Nek9em1flox/Cya
Common Name:
Nek9-flox
Product ID:
S-CKO-18490
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Nek9-flox
Strain ID
CKOCMP-217718-Nek9-B6J-VB
Gene Name
Nek9
Product ID
S-CKO-18490
Gene Alias
C130021H08Rik
Background
C57BL/6JCya
NCBI ID
217718
Modification
Conditional knockout
Chromosome
12
Phenotype
MGI:2387995
Document
Click here to download >>
Application
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More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nek9em1flox/Cya mice (Catalog S-CKO-18490) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040992
NCBI RefSeq
NM_145138
Target Region
Exon 3~4
Size of Effective Region
~1.2 kb
Detailed Document
Click here to download >>
Overview of Gene Research
NEK9, or NIMA-related kinase 9, is involved in multiple biological functions. It plays a role in spindle assembly, controlling chromosome alignment and centrosome separation [4,7]. It is also part of pathways related to ciliogenesis, cell-cycle progression, and autophagy. NEK9 is crucial in the microtubule polymerization, chromosome alignment, and mitosis as part of the NEK9-EG5 axis [3].

In terms of core findings, in mice, mutation in the LC3-interacting region (LIR) of NEK9 impairs in vivo cilia formation in the kidneys, suggesting that NEK9 regulates ciliogenesis by acting as an autophagy adaptor for MYH9, which inhibits ciliogenesis through actin network stabilization [1]. In breast cancer, immunohistochemistry analysis shows decreased Nek9 expression in invasive ductal carcinoma compared to benign breast lesions, and this decrease is associated with larger tumor size, high grade, and high Ki-67 index [4]. In gastric cancer, NEK9 directly regulates cell motility and RhoA activation by phosphorylating ARHGEF2, and is regulated by miR-520f-3p, which is transcriptionally suppressed by IL-6-mediated activation of STAT3 [2]. In colon cancer, overexpression of the NEK9-EG5 axis is associated with distant metastasis [3]. In pancreatic cancer, USP19 stabilizes NEK9 via deubiquitination, and NEK9 phosphorylates Raptor to inhibit the mTORC1 signaling pathway, leading to autophagic cell death [5]. In gastric cancer, CAF-derived SLIT2 activates NEK9, which phosphorylates TRIM28 and CTTN, inducing cytoskeletal reorganization and cell metastasis [6]. In p53-inactivated cancer cells, depletion of NEK9 selectively inhibits proliferation both in vitro and in vivo, causing cell-cycle arrest in G1 phase with senescence-like features [8]. Also, three novel pathogenic variants of NEK9 were detected in neonatal patients with arthrogryposis [9].

In conclusion, NEK9 is essential for various biological processes such as ciliogenesis, cell-cycle regulation, and autophagy. Model-based research, especially in mouse models, has revealed its significant roles in diseases including kidney cilia-related disorders, breast, gastric, colon, pancreatic cancers, and in p53-related cancer cell proliferation as well as neonatal arthrogryposis. Understanding NEK9's functions provides insights into disease mechanisms and potential therapeutic targets.

References:
1. Yamamoto, Yasuhiro, Chino, Haruka, Tsukamoto, Satoshi, Ueda, Hiroki R, Mizushima, Noboru. 2021. NEK9 regulates primary cilia formation by acting as a selective autophagy adaptor for MYH9/myosin IIA. In Nature communications, 12, 3292. doi:10.1038/s41467-021-23599-7. https://pubmed.ncbi.nlm.nih.gov/34078910/
2. Lu, Guofang, Tian, Siyuan, Sun, Yi, Feng, Bin, Shang, Yulong. 2021. NEK9, a novel effector of IL-6/STAT3, regulates metastasis of gastric cancer by targeting ARHGEF2 phosphorylation. In Theranostics, 11, 2460-2474. doi:10.7150/thno.53169. https://pubmed.ncbi.nlm.nih.gov/33500736/
3. Kim, Meejeong, Jeong, Hui Jeong, Ju, Hyun-Min, Jang, Se Jin, Choi, Jene. 2023. Overexpression of the NEK9-EG5 axis is a novel metastatic marker in pathologic stage T3 colon cancer. In Scientific reports, 13, 342. doi:10.1038/s41598-022-26249-0. https://pubmed.ncbi.nlm.nih.gov/36611072/
4. Xu, Ziru, Shen, Wenping, Pan, Aifeng, Gao, Peng, Li, Li. 2020. Decreased Nek9 expression correlates with aggressive behaviour and predicts unfavourable prognosis in breast cancer. In Pathology, 52, 329-335. doi:10.1016/j.pathol.2019.11.008. https://pubmed.ncbi.nlm.nih.gov/32098687/
5. Wang, Guangfu, Dai, Shangnan, Chen, Jin, Miao, Yi, Lu, Zipeng. 2024. USP19 potentiates autophagic cell death via inhibiting mTOR pathway through deubiquitinating NEK9 in pancreatic cancer. In Cell death and differentiation, 32, 702-713. doi:10.1038/s41418-024-01426-y. https://pubmed.ncbi.nlm.nih.gov/39627360/
6. Lu, Guofang, Du, Rui, Dong, Jiaqiang, Han, Ying, Shang, Yulong. 2023. Cancer associated fibroblast derived SLIT2 drives gastric cancer cell metastasis by activating NEK9. In Cell death & disease, 14, 421. doi:10.1038/s41419-023-05965-z. https://pubmed.ncbi.nlm.nih.gov/37443302/
7. Sdelci, Sara, Bertran, M Teresa, Roig, Joan. 2011. Nek9, Nek6, Nek7 and the separation of centrosomes. In Cell cycle (Georgetown, Tex.), 10, 3816-7. doi:10.4161/cc.10.22.18226. https://pubmed.ncbi.nlm.nih.gov/22064517/
8. Kurioka, Daisuke, Takeshita, Fumitaka, Tsuta, Koji, Kohno, Takashi, Tsuchiya, Naoto. 2014. NEK9-dependent proliferation of cancer cells lacking functional p53. In Scientific reports, 4, 6111. doi:10.1038/srep06111. https://pubmed.ncbi.nlm.nih.gov/25131192/
9. Liu, Fang, Dai, Liying, Li, Zhi, Yin's, Xiaowei. 2023. Novel variants of NEK9 associated with neonatal arthrogryposis: Two case reports and a literature review. In Frontiers in genetics, 13, 989215. doi:10.3389/fgene.2022.989215. https://pubmed.ncbi.nlm.nih.gov/36712877/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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