C57BL/6JCya-Ncoa2em1flox/Cya
Common Name:
Ncoa2-flox
Product ID:
S-CKO-18498
Background:
C57BL/6JCya
Product Type
Age
Genotype
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Basic Information
Strain Name
Ncoa2-flox
Strain ID
CKOCMP-17978-Ncoa2-B6J-VB
Gene Name
Product ID
S-CKO-18498
Gene Alias
D1Ertd433e; GRIP-1; Grip1; KAT13C; NCoA-2; SRC-2; TIF2; bHLHe75
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ncoa2em1flox/Cya mice (Catalog S-CKO-18498) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000006037
NCBI RefSeq
NM_008678.3
Target Region
Exon 4
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Ncoa2, also known as Nuclear receptor coactivator 2, is a member of the Ncoa family of coactivators. It serves as a transcription coactivator, regulating the activation and inhibition of transcription factors and proteins like those in the basic helix-loop-helix Per-ARNT-SIM family. It is involved in multiple biological processes and signaling pathways, with its dysregulation linked to various diseases [2,3]. Genetic models, such as KO/CKO mouse models, are valuable tools for studying its functions.
In T cells, Ncoa2-deficient mice (Ncoa2fl/fl/CD4Cre) had defective immune responses against implanted MC38 tumors, with reduced tumor-infiltrating CD8+ T cells and IFNγ production. CD8+ T cells from these mice also failed to reject tumors after adoptive transfer. Mechanically, TCR-stimulated Ncoa2-deficient CD8+ T cells had impaired mitochondrial function due to lower PGC-1α expression, as T-cell activation-induced phosphorylation of CREB recruits Ncoa2 to enhance PGC-1α expression [2]. In HEY1-NCOA2-induced mesenchymal chondrosarcoma mouse models, the fusion gene modulated chondrogenic differentiation. Treatment with the HDAC inhibitor panobinostat suppressed tumor growth, affecting genes downstream of HEY1-NCOA2 and Runx2 [1].
In conclusion, Ncoa2 is crucial in regulating T-cell-mediated antitumor immunity by enhancing mitochondrial function in CD8+ T cells and in modulating chondrogenic differentiation. Mouse models, especially KO/CKO models, have been instrumental in revealing its role in tumor-related immune responses and mesenchymal chondrosarcoma development, providing potential therapeutic insights for these disease areas.
References:
1. Tanaka, Miwa, Homme, Mizuki, Teramura, Yasuyo, Maruyama, Reo, Nakamura, Takuro. 2023. HEY1-NCOA2 expression modulates chondrogenic differentiation and induces mesenchymal chondrosarcoma in mice. In JCI insight, 8, . doi:10.1172/jci.insight.160279. https://pubmed.ncbi.nlm.nih.gov/37212282/
2. Zhong, Xiancai, Wu, Hongmin, Ouyang, Ching, Shang, Weirong, Sun, Zuoming. . Ncoa2 Promotes CD8+ T cell-Mediated Antitumor Immunity by Stimulating T-cell Activation via Upregulation of PGC-1α Critical for Mitochondrial Function. In Cancer immunology research, 11, 1414-1431. doi:10.1158/2326-6066.CIR-23-0092. https://pubmed.ncbi.nlm.nih.gov/37540802/
3. Tsai, Chi-Hao, Li, Ching-Hao, Liao, Po-Lin, Huang, Shih-Hsuan, Kang, Jaw-Jou. 2015. NcoA2-Dependent Inhibition of HIF-1α Activation Is Regulated via AhR. In Toxicological sciences : an official journal of the Society of Toxicology, 148, 517-30. doi:10.1093/toxsci/kfv199. https://pubmed.ncbi.nlm.nih.gov/26350169/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen