C57BL/6JCya-Ppp1r14bem1flox/Cya
Common Name:
Ppp1r14b-flox
Product ID:
S-CKO-18504
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ppp1r14b-flox
Strain ID
CKOCMP-18938-Ppp1r14b-B6J-VB
Gene Name
Product ID
S-CKO-18504
Gene Alias
AOM172; PHI-1; PLCB3N; Png
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ppp1r14bem1flox/Cya mice (Catalog S-CKO-18504) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000070850
NCBI RefSeq
NM_008889
Target Region
Exon 2~4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Ppp1r14b, protein phosphatase 1 regulatory subunit 14B, is a member of the protein phosphatase 1 regulatory subunits. It can inhibit protein phosphatase 1 (PP1) and different PP1 holoenzymes, which are important in regulating cell growth, the cell cycle, and apoptosis [2].
Gain-and loss-of-function assays in multiple cancer cell lines have demonstrated significant roles of Ppp1r14b. In triple-negative breast cancer, knockdown of Ppp1r14b inhibited cell proliferation, colony formation, migration, invasion, and increased sensitivity to paclitaxel both in vitro and in vivo. Mechanistically, it maintains phosphorylation and stability of oncoprotein stathmin 1 (STMN1), which is crucial for cancer progression and paclitaxel resistance [1]. Similar tumor-promoting effects were seen in other cancers. For example, in hepatocellular carcinoma, knockdown of Ppp1r14b significantly inhibited cell survival and tumorigenic ability, while overexpression had opposite effects. It regulated the stability and phosphorylation of RPS6KA1 and activated the AKT/NF-κB pathway [3]. In cervical and endometrial cancers, silencing Ppp1r14b constrained cell proliferation and promoted cell death by suppressing the activation of the Akt pathway [4].
In conclusion, Ppp1r14b plays a tumor-promoting role in multiple cancers, including triple-negative breast cancer, hepatocellular carcinoma, cervical and endometrial cancers. Through loss-of-function experiments in cell lines, its key role in promoting cancer cell proliferation, migration, invasion and drug resistance has been revealed. Understanding the function of Ppp1r14b provides potential therapeutic targets for these cancers.
References:
1. Liao, Li, Zhang, Yin-Ling, Deng, Ling, Shao, Zhi-Min, Li, Da-Qiang. . Protein Phosphatase 1 Subunit PPP1R14B Stabilizes STMN1 to Promote Progression and Paclitaxel Resistance in Triple-Negative Breast Cancer. In Cancer research, 83, 471-484. doi:10.1158/0008-5472.CAN-22-2709. https://pubmed.ncbi.nlm.nih.gov/36484700/
2. He, Kang, Wang, Taiwei, Huang, Xuemiao, Jiang, Junjie, Zhao, Lijing. 2023. PPP1R14B is a diagnostic prognostic marker in patients with uterine corpus endometrial carcinoma. In Journal of cellular and molecular medicine, 27, 846-863. doi:10.1111/jcmm.17697. https://pubmed.ncbi.nlm.nih.gov/36824011/
3. Zhou, Nana, Guo, Chaoqin, Du, Jingyang, Zheng, Xiaoliang, Tu, Linglan. 2024. PPP1R14B-mediated phosphorylation enhances protein stability of RPS6KA1 to promote hepatocellular carcinoma tumorigenesis. In Biochimica et biophysica acta. Molecular cell research, 1871, 119840. doi:10.1016/j.bbamcr.2024.119840. https://pubmed.ncbi.nlm.nih.gov/39216602/
4. Xiang, Nan, Chen, Tao, Zhao, Xiaoli, Zhao, Min. 2022. In vitro assessment of roles of PPP1R14B in cervical and endometrial cancer. In Tissue & cell, 77, 101845. doi:10.1016/j.tice.2022.101845. https://pubmed.ncbi.nlm.nih.gov/35679681/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen