C57BL/6JCya-Synj2em1flox/Cya
Common Name:
Synj2-flox
Product ID:
S-CKO-18518
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Synj2-flox
Strain ID
CKOCMP-20975-Synj2-B6J-VC
Gene Name
Product ID
S-CKO-18518
Gene Alias
SJ2; mKIAA0348
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Synj2em1flox/Cya mice (Catalog S-CKO-18518) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000115791
NCBI RefSeq
NM_001113353
Target Region
Exon 12~13
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Synj2, or synaptojanin 2, encodes an inositol polyphosphate phosphatase. It functions in recycling neurotransmitter vesicles and is implicated in spermatogenesis [8]. It also has a role in the regulation of cell proliferation and apoptosis via dephosphorylating plasma membrane phosphoinositides [10].
In various cancers, SYNJ2 shows significant associations. In lung squamous cell carcinoma (LUSC), it is upregulated at both mRNA and protein levels, predicting poor prognosis, and its cancer-promoting effect may be related to protein digestion and absorption and extracellular matrix-receptor interaction [1]. In papillary thyroid carcinoma (PTC), it is highly expressed, potentially promoting PTC progression through angiogenesis and epithelial-mesenchymal transition (EMT) pathways, and patients with high SYNJ2 expression show higher sensitivity to common drugs [3]. In gastric cancer, SYNJ2 promotes cell metastasis and angiogenesis by up-regulating GRB2 [4]. A SYNJ2 variant rs9365723 is associated with colorectal cancer risk, especially colon cancer [6]. In hepatocellular carcinoma (HCC), upregulated SYNJ2 can be used for predicting prognosis and may accelerate HCC occurrence and development via metabolic perturbation pathways [10]. In breast cancer, increased SYNJ2 in primary tumours correlates with invasive disease and reduced survival [9].
Also, in neurons, SYNJ2 is involved in transporting Pink1 mRNA to mitochondria for local mitophagy, and insulin signalling can regulate this process through AMPK-mediated phosphorylation of SYNJ2BP which interacts with SYNJ2 [2,5]. SYNJ2 is expressed in the murine small and large intestine, with its mRNA levels increasing with age in the colon, and may participate in intestinal pathology [7].
In conclusion, SYNJ2 is an important gene involved in multiple biological functions and disease processes. Its role in cancer, neuronal mitophagy, and intestinal functions has been revealed through various studies. Understanding SYNJ2 can provide insights into disease mechanisms and potential therapeutic targets.
References：
1. Hou, Wei, Li, Guo-Sheng, Gao, Li, Xia, Shuang, Wei, Hong-Yu. 2022. SYNJ2 is a novel and potential biomarker for the prediction and treatment of cancers: from lung squamous cell carcinoma to pan-cancer. In BMC medical genomics, 15, 114. doi:10.1186/s12920-022-01266-0. https://pubmed.ncbi.nlm.nih.gov/35581615/
2. Harbauer, Angelika B, Hees, J Tabitha, Wanderoy, Simone, He, Zhigang, Schwarz, Thomas L. 2022. Neuronal mitochondria transport Pink1 mRNA via synaptojanin 2 to support local mitophagy. In Neuron, 110, 1516-1531.e9. doi:10.1016/j.neuron.2022.01.035. https://pubmed.ncbi.nlm.nih.gov/35216662/
3. Yang, Yuan-Ping, Huang, Zhi-Guang, Luo, Jia-Yuan, Tang, Yi-Jun, Pang, Yu-Yan. 2024. Comprehensive transcriptome and scRNA-seq analyses uncover the expression and underlying mechanism of SYNJ2 in papillary thyroid carcinoma. In IET systems biology, 18, 183-198. doi:10.1049/syb2.12099. https://pubmed.ncbi.nlm.nih.gov/39370684/
4. Ning, Weiwei, Yang, Qingxu, Li, Zhengbiao, Xie, Ming. 2024. The activation of SYNJ2/GRB2 axis accelerates the malignant metastasis and angiogenesis of gastric cancer cells. In Molecular and cellular probes, 78, 101990. doi:10.1016/j.mcp.2024.101990. https://pubmed.ncbi.nlm.nih.gov/39521152/
5. Hees, J Tabitha, Wanderoy, Simone, Lindner, Jana, Murali Mahadevan, Hariharan, Harbauer, Angelika B. 2024. Insulin signalling regulates Pink1 mRNA localization via modulation of AMPK activity to support PINK1 function in neurons. In Nature metabolism, 6, 514-530. doi:10.1038/s42255-024-01007-w. https://pubmed.ncbi.nlm.nih.gov/38504131/
6. Du, Qingguo, Guo, Xueyan, Zhang, Xiyang, Jin, Tianbo, Liu, Chang. 2016. SYNJ2 variant rs9365723 is associated with colorectal cancer risk in Chinese Han population. In The International journal of biological markers, 31, e138-43. doi:10.5301/jbm.5000182. https://pubmed.ncbi.nlm.nih.gov/26616230/
7. Vázquez-Carretero, María Dolores, Carvajal, Ana Eloisa, Serrano-Morales, José Manuel, Ilundain, Anunciación Ana, Peral, María José. 2016. The Synaptojanins in the murine small and large intestine. In Journal of bioenergetics and biomembranes, 48, 569-579. doi:. https://pubmed.ncbi.nlm.nih.gov/27878645/
8. Planchart, Antonio. 2013. Analysis of an intronic promoter within Synj2. In Biochemical and biophysical research communications, 440, 640-5. doi:10.1016/j.bbrc.2013.09.115. https://pubmed.ncbi.nlm.nih.gov/24103750/
9. Csolle, Mariah P, Ooms, Lisa M, Papa, Antonella, Mitchell, Christina A. 2020. PTEN and Other PtdIns(3,4,5)P3 Lipid Phosphatases in Breast Cancer. In International journal of molecular sciences, 21, . doi:10.3390/ijms21239189. https://pubmed.ncbi.nlm.nih.gov/33276499/
10. Zhang, Rui, Mo, Wei-Jia, Huang, Lan-Shan, He, Wei-Ying, Feng, Zhen-Bo. . Identifying the Prognostic Risk Factors of Synaptojanin 2 and Its Underlying Perturbations Pathways in Hepatocellular Carcinoma. In Bioengineered, 12, 855-874. doi:10.1080/21655979.2021.1890399. https://pubmed.ncbi.nlm.nih.gov/33641617/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen