C57BL/6JCya-Prkd1em1flox/Cya
Common Name:
Prkd1-flox
Product ID:
S-CKO-18519
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Prkd1-flox
Strain ID
CKOCMP-18760-Prkd1-B6J-VB
Gene Name
Product ID
S-CKO-18519
Gene Alias
PKD; PKD1; Pkcm; Prkcm; nPKC-D1; nPKC-mu
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prkd1em1flox/Cya mice (Catalog S-CKO-18519) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000002765
NCBI RefSeq
NM_008858
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Prkd1, also known as PKCμ, encodes a serine/threonine protein kinase. It is involved in multiple fundamental cellular functions and is associated with pathways like the KRas-NF-κB pathway in pancreatic cancer [2]. Prkd1 is crucial for normal development and disease processes in various tissues.
In mouse models, homozygous deletion of Prkd1 in a transgenic mouse model (Prkd1em1) led to complex congenital heart diseases (CHDs) such as atrioventricular septal defects and bicuspid aortic and pulmonary valves, and was lethal. Even heterozygotes showed cardiac differences, indicating its role in cardiogenesis [3]. In osteocytes, inhibition or genetic deletion of Prkd1 using an osteocyte-targeted (Dmp1-Cre) conditional knockout (CKO) mouse model slowed plasma membrane disruption (PMD) repair rate and impaired post-wounding cell survival, affecting in vivo bone anabolic responses to loading [1].
In conclusion, Prkd1 is essential for normal cardiac development and the repair of plasma membrane disruptions in osteocytes. The study of Prkd1 using gene knockout and conditional knockout mouse models has provided insights into its role in CHDs and bone-related biological processes, contributing to our understanding of the underlying mechanisms of these diseases.
References:
1. Tuladhar, Anik, Shaver, Joseph C, McGee, Wesley A, Hamrick, Mark W, McGee-Lawrence, Meghan E. 2024. Prkd1 regulates the formation and repair of plasma membrane disruptions (PMD) in osteocytes. In Bone, 186, 117147. doi:10.1016/j.bone.2024.117147. https://pubmed.ncbi.nlm.nih.gov/38866124/
2. Döppler, Heike, Panayiotou, Richard, Reid, Elizabeth M, Bastea, Ligia, Storz, Peter. 2016. The PRKD1 promoter is a target of the KRas-NF-κB pathway in pancreatic cancer. In Scientific reports, 6, 33758. doi:10.1038/srep33758. https://pubmed.ncbi.nlm.nih.gov/27649783/
3. Waheed-Ullah, Qazi, Wilsdon, Anna, Abbad, Aseel, Brook, J David, Loughna, Siobhan. 2024. Effect of deletion of the protein kinase PRKD1 on development of the mouse embryonic heart. In Journal of anatomy, 245, 70-83. doi:10.1111/joa.14033. https://pubmed.ncbi.nlm.nih.gov/38419169/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen