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C57BL/6JCya-Zrsr2em1flox/Cya
Common Name:
Zrsr2-flox
Product ID:
S-CKO-18524
Background:
C57BL/6JCya
Product Type
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Basic Information
Strain Name
Zrsr2-flox
Strain ID
CKOCMP-22184-Zrsr2-B6J-VB
Gene Name
Zrsr2
Product ID
S-CKO-18524
Gene Alias
35kDa; 5031411E02Rik; A230052C13Rik; U2af1-rs2; URP
Background
C57BL/6JCya
NCBI ID
22184
Modification
Conditional knockout
Chromosome
X
Phenotype
MGI:103287
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zrsr2em1flox/Cya mice (Catalog S-CKO-18524) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000112289
NCBI RefSeq
NM_009453.3
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Zrsr2, zinc finger CCCH-type, RNA binding motif and serine/arginine rich 2, is an essential splicing factor. It is part of both the major and minor spliceosomes, recognizing 3'-intron splice sites, especially crucial for U12-type introns which represent a small fraction of human introns. It is involved in RNA and protein processing pathways, and its proper function is of great biological importance [2].

Zrsr2 mutant mouse lines showed blood cell anomalies, and in some lines, oogenesis was blocked at the secondary follicle stage. RNA-seq indicated aberrant gene expression and alternative splicing events, with enrichment of U12-type intron retention in Zrsr2 mutant secondary follicles [1]. In zebrafish, zrsr2-knockout embryos displayed multiple developmental defects and died after 8 days post-fertilization, along with down-regulation of essential metabolic pathways and aberrant retention of minor introns in many genes [2]. In mice, concurrent Zrsr2 mutation and Tet2 loss promoted myelodysplastic neoplasm (MDS), presenting with peripheral blood cytopenia, splenomegaly, and multi-lineage dysplasia [3].

In conclusion, Zrsr2 is vital for splicing, especially of U12-type introns, and plays a significant role in follicular development, embryonic development, and hematopoiesis. The study of Zrsr2 using knockout mouse models has provided insights into its functions in these processes and its association with diseases like MDS [1,2,3].

References:
1. Gómez-Redondo, Isabel, Pericuesta, Eva, Navarrete-Lopez, Paula, Horiuchi, Keiko, Gutiérrez-Adán, Alfonso. 2022. Zrsr2 and functional U12-dependent spliceosome are necessary for follicular development. In iScience, 25, 103860. doi:10.1016/j.isci.2022.103860. https://pubmed.ncbi.nlm.nih.gov/35198906/
2. Weinstein, Rachel, Bishop, Kevin, Broadbridge, Elizabeth, Bresciani, Erica, Sood, Raman. 2022. Zrsr2 Is Essential for the Embryonic Development and Splicing of Minor Introns in RNA and Protein Processing Genes in Zebrafish. In International journal of molecular sciences, 23, . doi:10.3390/ijms231810668. https://pubmed.ncbi.nlm.nih.gov/36142581/
3. Garcia-Ruiz, Cristian, Martínez-Valiente, Cristina, Cordón, Lourdes, Gutiérrez-Adán, Alfonso, Sanjuan-Pla, Alejandra. 2022. Concurrent Zrsr2 mutation and Tet2 loss promote myelodysplastic neoplasm in mice. In Leukemia, 36, 2509-2518. doi:10.1038/s41375-022-01674-2. https://pubmed.ncbi.nlm.nih.gov/36030305/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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