C57BL/6JCya-Wdr70em1flox/Cya
Common Name:
Wdr70-flox
Product ID:
S-CKO-18526
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Wdr70-flox
Strain ID
CKOCMP-545085-Wdr70-B6J-VB
Gene Name
Product ID
S-CKO-18526
Gene Alias
4833422F06Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Wdr70em1flox/Cya mice (Catalog S-CKO-18526) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000045766
NCBI RefSeq
NM_001081402
Target Region
Exon 4
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Wdr70, without common aliases mentioned in the references, is significantly involved in maintaining genomic stability. It is associated with pathways related to histone modification, DNA repair, and cell cycle regulation. Its biological importance lies in ensuring chromatin homeostasis and genetic stability, making it a crucial gene for understanding cellular processes [1,2,3,4,5,6]. Genetic models, such as fission yeast and human cell lines, have been valuable in studying its functions.
In fission yeast, depletion of the Wdr70 gene leads to cell cycle delay, hypersensitivity to DNA damage reagents, and quick phenotypic changes. It has a strong genetic interaction with genes regulating checkpoint and homologous recombination (HR), pinpointing its function to DNA end resection. The function of Wdr70 is attributed to monoubiquitination of histone H2B (uH2B) near DNA double strand breaks (DSBs) [1].
In human cells, studies show that Wdr70 promotes H2BK120ub1 by interacting with the RNF20/40 complex, and is involved in POLE3-mediated DNA double-strand breaks repair. Wdr70 also controls the expression of DNA damage response (DDR) factors like BRCA1, BRCA2, and RAD51. Loss of Wdr70 disrupts homologous recombination and chromosomal stability, as seen in ovarian cancer studies where its mutations lead to DNA repair defects [2,4,5,6].
In conclusion, Wdr70 is essential for chromatin modulation, DNA repair, and maintaining genomic stability. Studies using model systems like fission yeast and human cell lines have revealed its role in processes related to cell cycle and DNA damage response, with implications for understanding diseases such as ovarian cancer where Wdr70 mutations can lead to genomic instability [1,2,4,5,6].
References:
1. Zeng, Ming, Tang, Zizhi, Guo, Liandi, Wang, Xiaojun, Liu, Cong. 2020. Wdr70 regulates histone modification and genomic maintenance in fission yeast. In Biochimica et biophysica acta. Molecular cell research, 1867, 118665. doi:10.1016/j.bbamcr.2020.118665. https://pubmed.ncbi.nlm.nih.gov/32007529/
2. Mao, Xiaobing, Wu, Jian, Zhang, Qin, Liu, Cong, Han, Junhong. 2023. Requirement of WDR70 for POLE3-mediated DNA double-strand breaks repair. In Science advances, 9, eadh2358. doi:10.1126/sciadv.adh2358. https://pubmed.ncbi.nlm.nih.gov/37682991/
3. Zeng, Ming, Ren, Laifeng, Mizuno, Ken'Ichi, Carr, Antony M, Liu, Cong. 2016. CRL4(Wdr70) regulates H2B monoubiquitination and facilitates Exo1-dependent resection. In Nature communications, 7, 11364. doi:10.1038/ncomms11364. https://pubmed.ncbi.nlm.nih.gov/27098497/
4. Mirman, Zachary, Sharma, Keshav, Carroll, Thomas S, de Lange, Titia. 2022. Expression of BRCA1, BRCA2, RAD51, and other DSB repair factors is regulated by CRL4WDR70. In DNA repair, 113, 103320. doi:10.1016/j.dnarep.2022.103320. https://pubmed.ncbi.nlm.nih.gov/35316728/
5. Guo, Lian-di, Wang, Dan, Yang, Fan, Lou, Jiang-Yan, Chen, Jie. . [Functional Analysis of DNA Damage Repair Factor WDR70 and Its Mutation in Ovarian Cancer]. In Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 47, 501-506. doi:. https://pubmed.ncbi.nlm.nih.gov/28591950/
6. Tang, Zi-Zhi, Wang, Hai-Bin, Zeng, Ming, Liu, Cong, Li, De-Hua. . [DNA Repair Function and Mutation of an H2B Monoubiquitination Factor WDR70 in Ovarian Cancer]. In Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 48, 693-698. doi:. https://pubmed.ncbi.nlm.nih.gov/29130659/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen