C57BL/6JCya-Ddb2em1flox/Cya
Common Name
Ddb2-flox
Product ID
S-CKO-18555
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-107986-Ddb2-B6J-VB
When using this mouse strain in a publication, please cite “Ddb2-flox Mouse (Catalog S-CKO-18555) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ddb2-flox
Strain ID
CKOCMP-107986-Ddb2-B6J-VB
Gene Name
Product ID
S-CKO-18555
Gene Alias
2610043A19Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000028696
NCBI RefSeq
NM_028119
Target Region
Exon 5~7
Size of Effective Region
~1.7 kb
Overview of Gene Research
Ddb2, also known as damage-specific DNA-binding protein 2, is initially identified as a DNA damage recognition factor crucial for global genomic nucleotide excision repair (GG-NER) in human cells. It also involves in multiple essential biological processes such as chromatin remodeling, gene transcription, cell cycle regulation, and protein decay [1].
In cancer research, Ddb2 shows diverse roles. In pancreatic ductal adenocarcinoma (PDAC), its reduced expression in cancer tissues is related to shorter disease-free survival. Overexpression of Ddb2 in PDAC cells can repress epithelial-to-mesenchymal transition (EMT), inhibit cell migration and invasion, and sensitize cells to chemotherapy drugs like 5-fluorouracil, oxaliplatin, and gemcitabine, indicating its potential as a tumor suppressor in PDAC [2]. In melanoma, Ddb2 promotes cell growth by transcriptionally regulating the expression of KMT2A, and high Ddb2 levels predict a poor prognosis [3].
In conclusion, Ddb2 is vital for DNA repair and various biological processes. Its role in cancer development, such as in PDAC and melanoma, has been revealed through functional studies. These findings from model-based research on Ddb2 contribute to understanding the mechanisms of cancer development and may provide new therapeutic strategies for related cancers.
References：
1. Gilson, Pauline, Drouot, Guillaume, Witz, Andréa, Becuwe, Philippe, Harlé, Alexandre. 2019. Emerging Roles of DDB2 in Cancer. In International journal of molecular sciences, 20, . doi:10.3390/ijms20205168. https://pubmed.ncbi.nlm.nih.gov/31635251/
2. Dardare, Julie, Witz, Andréa, Betz, Margaux, Harlé, Alexandre, Gilson, Pauline. 2022. DDB2 represses epithelial-to-mesenchymal transition and sensitizes pancreatic ductal adenocarcinoma cells to chemotherapy. In Frontiers in oncology, 12, 1052163. doi:10.3389/fonc.2022.1052163. https://pubmed.ncbi.nlm.nih.gov/36568213/
3. Zhang, Changlin, Li, Weizhao, Liu, Lixiang, Huang, Jiajia, Li, Tian. . DDB2 promotes melanoma cell growth by transcriptionally regulating the expression of KMT2A and predicts a poor prognosis. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 38, e23735. doi:10.1096/fj.202302040R. https://pubmed.ncbi.nlm.nih.gov/38860936/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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