C57BL/6JCya-Incenpem1flox/Cya
Common Name
Incenp-flox
Product ID
S-CKO-18567
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-16319-Incenp-B6J-VB
Status
When using this mouse strain in a publication, please cite “Incenp-flox Mouse (Catalog S-CKO-18567) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Incenp-flox
Strain ID
CKOCMP-16319-Incenp-B6J-VB
Gene Name
Product ID
S-CKO-18567
Gene Alias
C130081E20, 2700067E22Rik
Background
C57BL/6JCya
Gene Full Name
inner centromere protein
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 19 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000025562
NCBI Transcript ID
NM_016692
Target Region
Exon 4
Size of Effective Region
~1.3 kb
Overview of Gene Research
Incenp, or inner centromere protein, is crucial for correct chromosome segregation during mitosis. It is part of the chromosomal passenger complex (CPC), which plays a vital role in multiple mitotic processes. The ATM-Chk2-INCENP pathway is involved in activating the abscission checkpoint, ensuring proper cytokinesis and preventing chromosome breakage or tetraploidization [1,6].
Phosphorylation of INCENP's intrinsically disordered region affects its conformational ensemble, influencing its cohesiveness and phase behavior, which is relevant for its function in the CPC [2]. INCENP paralogs in Oikopleura dioica show distinct localizations and subfunctionalization, regulating CPC functions during embryonic divisions [3]. INCENP binds directly to tubulin, and dynamic microtubules are required for its targeting to the cleavage furrow [4]. It is also essential for proper targeting of Survivin to the centromeres and anaphase spindle [5]. INCENP-aurora B interactions modulate kinase activity and CPC localization [7], and its localization at centromeres/inner kinetochores is required for chromosome bi-orientation in budding yeast [8]. Additionally, INCENP binds the Aurora-related kinase AIRK2 and is needed to target it to chromosomes, the central spindle, and cleavage furrow [9].
In conclusion, Incenp is integral to mitotic processes such as chromosome segregation, cytokinesis, and regulation of kinase activity. Understanding Incenp's functions through model-based research, including potential KO/CKO mouse models (not explicitly detailed in provided references but generally relevant for such gene function studies), could provide insights into mitotic-related diseases and chromosomal instability.
References：
1. Petsalaki, Eleni, Zachos, George. . An ATM-Chk2-INCENP pathway activates the abscission checkpoint. In The Journal of cell biology, 220, . doi:10.1083/jcb.202008029. https://pubmed.ncbi.nlm.nih.gov/33355621/
2. Martin, Isabel M, Aponte-Santamaría, Camilo, Schmidt, Lisa, Musacchio, Andrea, Gräter, Frauke. 2021. Phosphorylation tunes elongation propensity and cohesiveness of INCENP's intrinsically disordered region. In Journal of molecular biology, 434, 167387. doi:10.1016/j.jmb.2021.167387. https://pubmed.ncbi.nlm.nih.gov/34883116/
3. Feng, Haiyang, Raasholm, Martina, Moosmann, Alexandra, Campsteijn, Coen, Thompson, Eric M. 2019. Switching of INCENP paralogs controls transitions in mitotic chromosomal passenger complex functions. In Cell cycle (Georgetown, Tex.), 18, 2006-2025. doi:10.1080/15384101.2019.1634954. https://pubmed.ncbi.nlm.nih.gov/31306061/
4. Wheatley, S P, Kandels-Lewis, S E, Adams, R R, Ainsztein, A M, Earnshaw, W C. . INCENP binds directly to tubulin and requires dynamic microtubules to target to the cleavage furrow. In Experimental cell research, 262, 122-7. doi:. https://pubmed.ncbi.nlm.nih.gov/11139336/
5. Wheatley, S P, Carvalho, A, Vagnarelli, P, Earnshaw, W C. . INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis. In Current biology : CB, 11, 886-90. doi:. https://pubmed.ncbi.nlm.nih.gov/11516652/
6. Petsalaki, Eleni, Zachos, George. 2021. An ATM-CHK2-INCENP pathway prevents chromatin breakage by regulating the abscission checkpoint. In Molecular & cellular oncology, 8, 1877999. doi:10.1080/23723556.2021.1877999. https://pubmed.ncbi.nlm.nih.gov/33860082/
7. Xu, Zhenjie, Ogawa, Hiromi, Vagnarelli, Paola, Earnshaw, William C, Samejima, Kumiko. . INCENP-aurora B interactions modulate kinase activity and chromosome passenger complex localization. In The Journal of cell biology, 187, 637-53. doi:10.1083/jcb.200906053. https://pubmed.ncbi.nlm.nih.gov/19951914/
8. García-Rodríguez, Luis J, Kasciukovic, Taciana, Denninger, Viola, Tanaka, Tomoyuki U. 2019. Aurora B-INCENP Localization at Centromeres/Inner Kinetochores Is Required for Chromosome Bi-orientation in Budding Yeast. In Current biology : CB, 29, 1536-1544.e4. doi:10.1016/j.cub.2019.03.051. https://pubmed.ncbi.nlm.nih.gov/31006569/
9. Adams, R R, Wheatley, S P, Gouldsworthy, A M, Gerloff, D L, Earnshaw, W C. . INCENP binds the Aurora-related kinase AIRK2 and is required to target it to chromosomes, the central spindle and cleavage furrow. In Current biology : CB, 10, 1075-8. doi:. https://pubmed.ncbi.nlm.nih.gov/10996078/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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