C57BL/6JCya-Mnx1em1flox/Cya
Common Name:
Mnx1-flox
Product ID:
S-CKO-18569
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mnx1-flox
Strain ID
CKOCMP-15285-Mnx1-B6J-VB
Gene Name
Product ID
S-CKO-18569
Gene Alias
HB9; Hlxb9; MNR2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mnx1em1flox/Cya mice (Catalog S-CKO-18569) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000165512
NCBI RefSeq
NM_019944
Target Region
Exon 2~3
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Mnx1, also known as Motor neuron and pancreas homeobox 1, encodes a homeobox transcription factor. It is crucial for pancreatic beta cell differentiation and development, and is involved in pathways related to cell development and potentially tumorigenesis [1]. Genetic models, such as gene knockout (KO) or conditional knockout (CKO) mouse models, can be valuable for studying its function.
Mutations of the MNX1 gene can cause permanent neonatal diabetes mellitus (PNDM). A case of a preterm male infant with a homozygous MNX1 mutation presented in severe diabetic ketoacidosis and had extra-pancreatic features like hypospadias and congenital umbilical hernia, suggesting a variable phenotype associated with MNX1 mutations [1]. In lung adenocarcinoma, MNX1 knockdown significantly suppressed cell proliferation, migration, invasion, hindered cell cycle progression, promoted apoptosis in vitro, and inhibited tumor growth in vivo, as it directly binds to the CCDC34 promoter and transcriptionally activates CCDC34 expression [2].
In conclusion, Mnx1 is essential for pancreatic beta cell development. Model-based research, especially KO mouse models, has revealed its role in neonatal diabetes and lung adenocarcinoma. Understanding Mnx1 provides insights into disease mechanisms, potentially leading to new treatment strategies for these conditions.
References:
1. Aly, Hanan Hassan, De Franco, Elisa, Flanagan, Sarah E, Elhenawy, Yasmine I. 2022. MNX1 mutations causing neonatal diabetes: Review of the literature and report of a case with extra-pancreatic congenital defects presenting in severe diabetic ketoacidosis. In Journal of diabetes investigation, 14, 516-521. doi:10.1111/jdi.13968. https://pubmed.ncbi.nlm.nih.gov/36586106/
2. Wu, Junhua, Yue, Chongmei, Xu, Weiguo, Zhu, Jing, Li, Lin. 2023. MNX1 facilitates the malignant progress of lung adenocarcinoma through transcriptionally upregulating CCDC34. In Oncology letters, 26, 325. doi:10.3892/ol.2023.13911. https://pubmed.ncbi.nlm.nih.gov/37415626/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen