C57BL/6JCya-G2e3em1flox/Cya
Common Name
G2e3-flox
Product ID
S-CKO-18574
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-217558-G2e3-B6J-VB
Status
When using this mouse strain in a publication, please cite “G2e3-flox Mouse (Catalog S-CKO-18574) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
G2e3-flox
Strain ID
CKOCMP-217558-G2e3-B6J-VB
Gene Name
Product ID
S-CKO-18574
Gene Alias
mKIAA1333, 9030416F18, 6030408C04Rik, D930034K21Rik
Background
C57BL/6JCya
Gene Full Name
G2/M-phase specific E3 ubiquitin ligase
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 12 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000119211
NCBI Transcript ID
NM_001167963
Target Region
Exon 3~4
Size of Effective Region
~1.8 kb
Overview of Gene Research
G2E3, a G2/M-phase-specific E3 ubiquitin protein ligase, contains a conserved HECT domain and is likely to function as a ubiquitin ligase. It is a nucleo-cytoplasmic shuttling protein with a novel nuclear export domain and a nucleolar localization signal, suggesting its role in cell cycle regulation and the cellular response to DNA damage [1]. It also has been implicated in early embryonic development, preventing apoptotic death [2].
G2E3 knockout (KO) mouse models have provided valuable insights. G2E3 null embryos die prior to implantation due to massive apoptosis in blastocysts [2]. G2e3 KO mice also exhibit increased body fat, obesity, and glucose intolerance, suggesting that obesity-associated variants near human G2E3 may decrease G2E3 activity [4]. In breast cancer, high G2E3 expression is associated with worse survival outcomes, and genes co-expressed with G2E3 may be enriched in cancer-related signaling pathways like E2F, PI3K/AKT/mTOR, and DNA repair pathways [3]. Loss of G2E3 sensitizes tumor cells to chemotherapy, as it leads to decreased DNA damage response markers and increased apoptosis and decreased proliferation of cancer cells [5].
In summary, G2E3 is essential for early embryonic development by preventing apoptosis. Its dysregulation is associated with obesity and poor prognosis in breast cancer. The G2E3 KO mouse models have been crucial in uncovering these roles, highlighting its potential as a therapeutic target in cancer and a gene of interest in obesity-related research.
References：
1. Brooks, William S, Banerjee, Sami, Crawford, David F. 2006. G2E3 is a nucleo-cytoplasmic shuttling protein with DNA damage responsive localization. In Experimental cell research, 313, 665-76. doi:. https://pubmed.ncbi.nlm.nih.gov/17239372/
2. Brooks, William S, Helton, E Scott, Banerjee, Sami, Kesterson, Robert A, Crawford, David F. 2008. G2E3 is a dual function ubiquitin ligase required for early embryonic development. In The Journal of biological chemistry, 283, 22304-15. doi:10.1074/jbc.M803238200. https://pubmed.ncbi.nlm.nih.gov/18511420/
3. Shen, Yanyan, Xue, Jinqi, Yu, Jiahui, Gu, Xi, Zhu, Xudong. 2022. Comprehensive analysis of the expression, prognostic significance, and regulation pathway of G2E3 in breast cancer. In World journal of surgical oncology, 20, 398. doi:10.1186/s12957-022-02871-0. https://pubmed.ncbi.nlm.nih.gov/36517818/
4. Powell, David R, Doree, Deon D, DaCosta, Christopher M, Revelli, Jean-Pierre, Brommage, Robert. 2020. Obesity of G2e3 Knockout Mice Suggests That Obesity-Associated Variants Near Human G2E3 Decrease G2E3 Activity. In Diabetes, metabolic syndrome and obesity : targets and therapy, 13, 2641-2652. doi:10.2147/DMSO.S259546. https://pubmed.ncbi.nlm.nih.gov/32801815/
5. Schmidt, Franziska, Kunze, Meike, Loock, Ann-Christine, Dobbelstein, Matthias. . Screening analysis of ubiquitin ligases reveals G2E3 as a potential target for chemosensitizing cancer cells. In Oncotarget, 6, 617-32. doi:. https://pubmed.ncbi.nlm.nih.gov/25593194/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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