C57BL/6JCya-Hsf4em1flox/Cya
Common Name:
Hsf4-flox
Product ID:
S-CKO-18577
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Hsf4-flox
Strain ID
CKOCMP-26386-Hsf4-B6J-VA
Gene Name
Product ID
S-CKO-18577
Gene Alias
HSF 4; HSTF 4; HSTF4; ldis1; mHSF4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hsf4em1flox/Cya mice (Catalog S-CKO-18577) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000036127
NCBI RefSeq
NM_001256042
Target Region
Exon 2~9
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Hsf4, a transcription factor, is closely associated with multiple biological processes. Mutations in Hsf4 are responsible for congenital cataract formation, and it also plays roles in regulating heat stress response, autophagy, and tumor progression. It is involved in pathways related to cell stress reaction, organelle degradation, and cell cycle regulation [2,3,4]. Genetic models, such as knockout mouse models, have been crucial in studying its functions.
In maize, ZmHsf4 promotes the heat stress response, and its down-regulation by ZmHSF20 affects thermotolerance, placing ZmHsf4 downstream of ZmHSF20 [1]. In HSF4del42 mutant mice with congenital cataracts, HSF4 was found to facilitate organelle degradation by transcriptionally activating autophagy during lens terminal differentiation [2]. In colorectal cancer, HSF4 promotes tumor progression by transactivating c-MET, enhancing the activity of downstream ERK1/2 and AKT signaling pathways [3].
In conclusion, Hsf4 has diverse functions including promoting heat stress response in maize, facilitating autophagy during lens terminal differentiation, and promoting tumor progression in colorectal cancer. Studies using knockout models in mice and other organisms have been instrumental in uncovering these functions, contributing to our understanding of related biological processes and disease mechanisms.
References:
1. Li, Ze, Li, Zerui, Ji, Yulong, Le, Jie, Zhang, Mei. . The heat shock factor 20-HSF4-cellulose synthase A2 module regulates heat stress tolerance in maize. In The Plant cell, 36, 2652-2667. doi:10.1093/plcell/koae106. https://pubmed.ncbi.nlm.nih.gov/38573521/
2. Zhang, Jing, Jiang, Ning, Du, Chunxiao, Hu, Yanzhong, Cui, Xiukun. . HSF4 Transcriptionally Activates Autophagy by Regulating ATG9a During Lens Terminal Differentiation. In Investigative ophthalmology & visual science, 64, 5. doi:10.1167/iovs.64.7.5. https://pubmed.ncbi.nlm.nih.gov/37266953/
3. Zhang, Wenjing, Zhang, Xuelian, Cheng, Peng, Guo, Qiang, Zhang, Yu. 2022. HSF4 promotes tumor progression of colorectal cancer by transactivating c-MET. In Molecular and cellular biochemistry, 478, 1141-1150. doi:10.1007/s11010-022-04582-2. https://pubmed.ncbi.nlm.nih.gov/36229759/
4. Anand, Deepti, Agrawal, Smriti A, Slavotinek, Anne, Lachke, Salil A. 2018. Mutation update of transcription factor genes FOXE3, HSF4, MAF, and PITX3 causing cataracts and other developmental ocular defects. In Human mutation, 39, 471-494. doi:10.1002/humu.23395. https://pubmed.ncbi.nlm.nih.gov/29314435/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen