Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Ano3em1flox/Cya
Common Name:
Ano3-flox
Product ID:
S-CKO-18578
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ano3-flox
Strain ID
CKOCMP-228432-Ano3-B6J-VB
Gene Name
Ano3
Product ID
S-CKO-18578
Gene Alias
B230324K02Rik; Tmem16c
Background
C57BL/6JCya
NCBI ID
228432
Modification
Conditional knockout
Chromosome
2
Phenotype
MGI:3613666
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ano3em1flox/Cya mice (Catalog S-CKO-18578) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000099623
NCBI RefSeq
NM_001128103
Target Region
Exon 6
Size of Effective Region
~1.5 kb
Detailed Document
Click here to download >>
Overview of Gene Research
ANO3, a member of the anoctamin (TMEM16) protein family, is involved in functions such as ion transport and phospholipid scrambling [5]. It has been associated with the calcium homeostasis pathway, and its malfunction may disrupt normal cellular functions [3]. Mutations in ANO3 are relevant as they can lead to various genetic diseases, highlighting its biological importance [5].

Mutations in ANO3 are a rare cause of autosomal dominant isolated or combined dystonia, mainly presenting in adulthood. A study of a new ANO3 family found that a heterozygous p.G6V variant in ANO3 was identified in all affected subjects, with younger patients displaying a more severe phenotype, likely due to variants near the scrambling domain [1]. Another study described a 3-year-old girl with distal myoclonic dystonia and a de novo missense ANO3 variant, along with psychomotor regression and brain atrophy, suggesting that missense mutations in ANO3 may underlie complex disorders with early psychomotor regression and dystonia [2]. In a Chinese cohort, four different ANO3 variants were identified in dystonia patients, and a novel missense mutation in a teenaged girl with generalized dystonia was classified as "likely pathogenic" and she responded well to deep brain stimulation [4]. Overall, ANO3 mutations seem to expand the mutational and clinical spectrum of dystonia 24 (DYT24) [4].

In conclusion, ANO3 is crucial for normal cellular function through its role in ion transport and phospholipid scrambling. Studies on ANO3-related mutations, especially in the context of dystonia, have provided insights into the genotype-phenotype characteristics of the disorder. These findings contribute to understanding the pathogenesis of dystonia and may potentially lead to new treatment strategies for this movement disorder.

References:
1. Percetti, Marco, Zini, Michela, Soliveri, Paola, Isaias, Ioannis Ugo, Sacilotto, Giorgio. 2024. The Clinical Spectrum of ANO3-Report of a New Family and Literature Review. In Movement disorders clinical practice, 11, 289-297. doi:10.1002/mdc3.13979. https://pubmed.ncbi.nlm.nih.gov/38284143/
2. Jiménez de Domingo, Ana, Lopez-Martín, Sara, Albert, Jacobo, Álvarez, Sara, Fernández-Jaén, Alberto. 2020. ANO3 and early-onset dyskinetic encephalopathy. In European journal of medical genetics, 63, 104085. doi:10.1016/j.ejmg.2020.104085. https://pubmed.ncbi.nlm.nih.gov/33045406/
3. Thomsen, Mirja, Lange, Lara M, Zech, Michael, Lohmann, Katja. 2023. Genetics and Pathogenesis of Dystonia. In Annual review of pathology, 19, 99-131. doi:10.1146/annurev-pathmechdis-051122-110756. https://pubmed.ncbi.nlm.nih.gov/37738511/
4. Li, Shanglin, Wang, Lin, Yang, Yingmai, Ma, Jun, Wan, Xinhua. 2020. ANO3 Mutations in Chinese Dystonia: A Genetic Screening Study Using Next-Generation Sequencing. In Frontiers in neurology, 10, 1351. doi:10.3389/fneur.2019.01351. https://pubmed.ncbi.nlm.nih.gov/32116979/
5. Pedemonte, Nicoletta, Galietta, Luis J V. . Structure and function of TMEM16 proteins (anoctamins). In Physiological reviews, 94, 419-59. doi:10.1152/physrev.00039.2011. https://pubmed.ncbi.nlm.nih.gov/24692353/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest