C57BL/6JCya-Cpsf1em1flox/Cya
Common Name:
Cpsf1-flox
Product ID:
S-CKO-18597
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cpsf1-flox
Strain ID
CKOCMP-94230-Cpsf1-B6J-VA
Gene Name
Product ID
S-CKO-18597
Gene Alias
CPSF160
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cpsf1em1flox/Cya mice (Catalog S-CKO-18597) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000230157
NCBI RefSeq
NM_001164173
Target Region
Exon 2~25
Size of Effective Region
~5.6 kb
Detailed Document
Overview of Gene Research
Cpsf1, short for cleavage and polyadenylation specificity factor 1, is a crucial factor involved in 3' untranslated region processing of a subset of pre-mRNAs. It participates in various biological pathways, such as the MAPK/ERK pathway, and is associated with multiple biological processes including hematopoiesis, cell apoptosis, migration, and alternative polyadenylation. Genetic models, like zebrafish mutants, have been used to study its function [4].
In diabetes-related studies, down-regulation of Cpsf1 was observed in diabetic retinopathy tissues and high-glucose-induced human retinal vascular endothelial cells. Up-regulated Cpsf1 alleviated apoptosis and migration via the MAPK/ERK pathway, suggesting its role in the development of diabetic retinopathy [1]. In gastric cancer, Cpsf1 is significantly up-regulated, promoting cancer progression by positively regulating NSDHL through alternative polyadenylation [2]. In prostate cancer, high Cpsf1 expression correlates with worse progression-free survival, and its knockdown inhibits cell growth and glycolysis, while promoting the use of intergenic polyadenylation sites [3].
In conclusion, Cpsf1 plays essential roles in multiple biological processes and is associated with various diseases including diabetic retinopathy, gastric cancer, and prostate cancer. Studies using different genetic models have revealed its functions in these disease-related biological processes, providing insights into potential therapeutic targets.
References:
1. Zhang, Jingyi, Zhang, Xi, Zou, Yuanyuan, Han, Fengmei. 2020. CPSF1 mediates retinal vascular dysfunction in diabetes mellitus via the MAPK/ERK pathway. In Archives of physiology and biochemistry, 128, 708-715. doi:10.1080/13813455.2020.1722704. https://pubmed.ncbi.nlm.nih.gov/32046510/
2. Kang, Weibiao, Yang, Yang, Chen, Changyu, Yu, Changjun. 2022. CPSF1 positively regulates NSDHL by alternative polyadenylation and promotes gastric cancer progression. In American journal of cancer research, 12, 4566-4583. doi:. https://pubmed.ncbi.nlm.nih.gov/36381317/
3. Tietz, Kiel T, McCluskey, Braedan M, Miller, Conor R, Munro, Sarah A, Dehm, Scott M. 2025. CPSF1 inhibition promotes widespread use of intergenic polyadenylation sites and impairs glycolysis in prostate cancer cells. In Cell reports, 44, 115211. doi:10.1016/j.celrep.2024.115211. https://pubmed.ncbi.nlm.nih.gov/39847481/
4. Bolli, Niccolò, Payne, Elspeth M, Rhodes, Jennifer, Zon, Leonard I, Look, A Thomas. 2011. cpsf1 is required for definitive HSC survival in zebrafish. In Blood, 117, 3996-4007. doi:10.1182/blood-2010-08-304030. https://pubmed.ncbi.nlm.nih.gov/21330472/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen