C57BL/6JCya-Syt12em1flox/Cya
Common Name:
Syt12-flox
Product ID:
S-CKO-18598
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Syt12-flox
Strain ID
CKOCMP-171180-Syt12-B6J-VB
Gene Name
Product ID
S-CKO-18598
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Syt12em1flox/Cya mice (Catalog S-CKO-18598) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000059295
NCBI RefSeq
NM_134164
Target Region
Exon 4~6
Size of Effective Region
~5.2 kb
Detailed Document
Overview of Gene Research
Syt12, or Synaptotagmin 12, is a member of the synaptotagmins family. It is an abundant synaptic vesicle protein not binding Ca(2+), and is phosphorylated by cAMP-dependent protein kinase-A at serine-97 in an activity-dependent manner. It plays a role in cAMP-dependent synaptic plasticity, especially in hippocampal mossy fiber long-term potentiation (LTP) [5].
In cancer research, Syt12 has been shown to act as an oncogene in multiple cancer types. In thyroid carcinoma, its overexpression promotes cancer progression and metastasis, increasing the risk of lymph node metastatic and certain histological types of primary neoplasm [1]. In oral cancer, Syt12 is up-regulated, and its knockdown leads to depressed cellular proliferation, migration, and invasion with cell cycle arrest at G1 phase, potentially via the CAMK2N1 and CAMK2 pathway [2]. In gastric cancer, it may promote the proliferation and migration of cancer cells by inducing epithelial-mesenchymal transition (EMT) [3]. In lung adenocarcinoma, Syt12 expression is increased, associated with advanced tumor stage and reduced prognosis, and it promotes cell proliferation and migration through the PI3K/AKT/mTOR pathway [4].
In conclusion, Syt12 has a crucial function in cAMP-dependent synaptic plasticity in the nervous system. In cancer, it often acts as an oncogene promoting cancer cell proliferation, migration, and invasion in various cancer types such as thyroid, oral, gastric, and lung adenocarcinoma. The study of Syt12 using gene-knockout models in mice has helped to reveal its role in these biological processes and disease conditions, providing potential targets for cancer diagnosis and treatment.
References:
1. Jin, Lingli, Zheng, Danni, Chen, Danxiang, Bhandari, Adheesh, Wang, Ouchen. 2021. SYT12 is a novel oncogene that promotes thyroid carcinoma progression and metastasis. In Journal of Cancer, 12, 6851-6860. doi:10.7150/jca.62555. https://pubmed.ncbi.nlm.nih.gov/34659573/
2. Eizuka, Keitaro, Nakashima, Dai, Oka, Noritoshi, Tanzawa, Hideki, Uzawa, Katsuhiro. 2019. SYT12 plays a critical role in oral cancer and may be a novel therapeutic target. In Journal of Cancer, 10, 4913-4920. doi:10.7150/jca.32582. https://pubmed.ncbi.nlm.nih.gov/31598163/
3. Niu, Xingdong, Ma, Fubin, Li, Fangying, Wang, Junhong, Da, Mingxu. 2024. Integration of bioinformatics and cellular experiments unveils the role of SYT12 in gastric cancer. In BMC cancer, 24, 1331. doi:10.1186/s12885-024-13077-w. https://pubmed.ncbi.nlm.nih.gov/39472897/
4. Liu, Kaichao, Luo, Jing, Shao, Chenye, Wu, Haiwei, Shen, Yi. 2020. Synaptotagmin 12 (SYT12) Gene Expression Promotes Cell Proliferation and Progression of Lung Adenocarcinoma and Involves the Phosphoinositide 3-Kinase (PI3K)/AKT/Mammalian Target of Rapamycin (mTOR) Pathway. In Medical science monitor : international medical journal of experimental and clinical research, 26, e920351. doi:10.12659/MSM.920351. https://pubmed.ncbi.nlm.nih.gov/32108133/
5. Kaeser-Woo, Yea Jin, Younts, Thomas J, Yang, Xiaofei, Castillo, Pablo E, Südhof, Thomas C. . Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP. In The Journal of neuroscience : the official journal of the Society for Neuroscience, 33, 9769-80. doi:10.1523/JNEUROSCI.5814-12.2013. https://pubmed.ncbi.nlm.nih.gov/23739973/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen