C57BL/6JCya-Rpl3lem1flox/Cya
Common Name
Rpl3l-flox
Product ID
S-CKO-18603
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-66211-Rpl3l-B6J-VB
When using this mouse strain in a publication, please cite “Rpl3l-flox Mouse (Catalog S-CKO-18603) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Rpl3l-flox
Strain ID
CKOCMP-66211-Rpl3l-B6J-VB
Gene Name
Product ID
S-CKO-18603
Gene Alias
1110057H16Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 17
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000170239
NCBI RefSeq
NM_001163945
Target Region
Exon 4~7
Size of Effective Region
~2.6 kb
Overview of Gene Research
Rpl3l, ribosomal protein L3-like, is a paralog of RPL3 specifically expressed in heart and skeletal muscle. It is involved in ribosome-related functions, influencing translation elongation dynamics, which is crucial for normal cardiac function [1]. It may also be associated with pathways like those related to cardiac muscle contraction and dilated cardiomyopathy [1]. Mouse models are valuable for studying its function.
In Rpl3l knockout male mice, the deficiency of RPL3L-containing ribosomes led to impaired cardiac contractility and altered ribosome occupancy at mRNA codons [1].
Rpl3l gene-deleted mice showed no overt cardiac changes at baseline or after pressure overload hypertrophy, likely due to up-regulation of RPL3, but by 18 months, they had significantly smaller hearts [2].
Rpl3l knockout mice also demonstrated increased ribosome-mitochondria interactions in cardiomyocytes and higher ATP levels, suggesting modulation of mitochondrial activity [3].
In conclusion, Rpl3l is essential for normal cardiac function through its impact on translation elongation dynamics and mitochondrial activity. Mouse knockout models have been instrumental in revealing its role in cardiac-related diseases such as dilated cardiomyopathy and age-related changes in heart size [1,2,3].
References：
1. Shiraishi, Chisa, Matsumoto, Akinobu, Ichihara, Kazuya, Tsutsui, Hiroyuki, Nakayama, Keiichi I. 2023. RPL3L-containing ribosomes determine translation elongation dynamics required for cardiac function. In Nature communications, 14, 2131. doi:10.1038/s41467-023-37838-6. https://pubmed.ncbi.nlm.nih.gov/37080962/
2. Grimes, Kelly M, Prasad, Vikram, Huo, Jiuzhou, Lin, Suh-Chin J, Molkentin, Jeffery D. 2023. Rpl3l gene deletion in mice reduces heart weight over time. In Frontiers in physiology, 14, 1054169. doi:10.3389/fphys.2023.1054169. https://pubmed.ncbi.nlm.nih.gov/36733907/
3. Milenkovic, Ivan, Santos Vieira, Helaine Graziele, Lucas, Morghan C, Völkers, Mirko, Novoa, Eva Maria. . Dynamic interplay between RPL3- and RPL3L-containing ribosomes modulates mitochondrial activity in the mammalian heart. In Nucleic acids research, 51, 5301-5324. doi:10.1093/nar/gkad121. https://pubmed.ncbi.nlm.nih.gov/36882085/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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