C57BL/6JCya-Ggta1em1flox/Cya
Common Name:
Ggta1-flox
Product ID:
S-CKO-18622
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ggta1-flox
Strain ID
CKOCMP-14594-Ggta1-B6J-VB
Gene Name
Product ID
S-CKO-18622
Gene Alias
GALT; Gal; Ggta; Ggta-1; alpha Gal; alpha3GalT
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ggta1em1flox/Cya mice (Catalog S-CKO-18622) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102794
NCBI RefSeq
NM_010283.4
Target Region
Exon 4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Ggta1, encoding α1,3-galactosyltransferase, is crucial for the biosynthesis of galactosyl-alpha 1,3-galactose, the major xenoantigen causing hyperacute rejection in xenotransplantation [3]. It regulates the expression of the carbohydrate epitope Galα1-3Galβ1-4GlcNAc-R, known as the α-Gal antigen, which is a primary xenoantigen recognized by the human immune system [1].
In gene-editing studies, GGTA1-KO pigs and rabbits have been developed. In rabbits, the mutation efficiency of GGTA1 gene-editing using the Nuclease technology system was as high as 92.3% in F0 pups, and α-Gal antigen expression in major organs decreased by over 99.96% compared to wild-type rabbits [1]. In pigs, genetic knockout of GGTA1 removes the immunogenic α-Gal epitope, but may lead to the emergence of neo-glycans, changing the distribution and levels of other porcine glycans [2]. Also, in GGTA1-KO pigs, N-glycans capped with Neu5Gc were increased compared to wild-type pigs [2].
In conclusion, GGTA1 plays a central role in the immunogenicity of xenografts due to its role in synthesizing the α-Gal antigen. The generation of GGTA1-KO animal models, especially pigs and rabbits, has significantly contributed to understanding the immunological mechanisms in xenotransplantation, aiming to overcome the immunological rejection hurdles in pig-to-human organ transplantation [1,2,3].
References:
1. Wei, Lina, Mu, Yufeng, Deng, Jichao, Qu, Shuxin, Xu, Liming. 2022. α-Gal antigen-deficient rabbits with GGTA1 gene disruption via Nuclease technology. In BMC genomic data, 23, 54. doi:10.1186/s12863-022-01068-4. https://pubmed.ncbi.nlm.nih.gov/35820824/
2. Morticelli, Lucrezia, Rossdam, Charlotte, Cajic, Samanta, Buettner, Falk F R, Hilfiker, Andres. 2023. Genetic knockout of porcine GGTA1 or CMAH/GGTA1 is associated with the emergence of neo-glycans. In Xenotransplantation, 30, e12804. doi:10.1111/xen.12804. https://pubmed.ncbi.nlm.nih.gov/37148126/
3. Tanihara, Fuminori, Hirata, Maki, Nguyen, Nhien Thi, Doi, Masako, Otoi, Takeshige. 2020. Efficient generation of GGTA1-deficient pigs by electroporation of the Nuclease technology system into in vitro-fertilized zygotes. In BMC biotechnology, 20, 40. doi:10.1186/s12896-020-00638-7. https://pubmed.ncbi.nlm.nih.gov/32811500/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen