C57BL/6JCya-Dnajc5em1flox/Cya
Common Name
Dnajc5-flox
Product ID
S-CKO-18637
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-13002-Dnajc5-B6J-VB
When using this mouse strain in a publication, please cite “Dnajc5-flox Mouse (Catalog S-CKO-18637) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Dnajc5-flox
Strain ID
CKOCMP-13002-Dnajc5-B6J-VB
Gene Name
Product ID
S-CKO-18637
Gene Alias
2610314I24Rik, Csp
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000072334
NCBI RefSeq
NM_016775
Target Region
Exon 2~5
Size of Effective Region
~3.2 kb
Overview of Gene Research
Dnajc5, also known as cysteine tandem protein (CSPα), is a member of the DnaJ/Hsp40 family of co-chaperones. It is crucial for maintaining normal nerve tissue function. Palmitoylated Dnajc5 anchors on the membrane and is involved in unconventional protein secretion pathways, such as facilitating the secretion of α-synuclein [1]. It also safeguards lysosomal homeostasis through unconventional protein secretion and endosomal microautophagy, processes relevant to maintaining cellular health [2].
Mutations in Dnajc5 are associated with adult neuronal ceroid lipofuscinosis (ANCL), a neurodegenerative disease. In ANCL, abnormal triaging of misfolded proteins by Dnajc5 mutants causes lipofuscin accumulation. Uncoupling the two chaperoning activities of Dnajc5, which regulate lysosomal homeostasis, generates lipofuscin-like materials and induces neurodegeneration in a Drosophila ANCL model [3].
In conclusion, Dnajc5 is essential for maintaining lysosomal homeostasis and proper protein secretion, especially in nerve tissues. The study of Dnajc5 in genetic models like the Drosophila ANCL model has revealed its role in neurodegenerative diseases such as ANCL, providing insights into the underlying pathological mechanisms.
References：
1. Wu, Shenjie, Hernandez Villegas, Nancy C, Sirkis, Daniel W, Wade-Martins, Richard, Schekman, Randy. 2023. Unconventional secretion of α-synuclein mediated by palmitoylated DNAJC5 oligomers. In eLife, 12, . doi:10.7554/eLife.85837. https://pubmed.ncbi.nlm.nih.gov/36626307/
2. Lee, Juhyung, Xu, Yue, Ye, Yihong. 2022. Safeguarding Lysosomal Homeostasis by DNAJC5/CSPα-Mediated Unconventional Protein Secretion and Endosomal Microautophagy. In Frontiers in cell and developmental biology, 10, 906453. doi:10.3389/fcell.2022.906453. https://pubmed.ncbi.nlm.nih.gov/35620055/
3. Lee, Juhyung, Xu, Yue, Saidi, Layla, Zinsmaier, Konrad, Ye, Yihong. 2022. Abnormal triaging of misfolded proteins by adult neuronal ceroid lipofuscinosis-associated DNAJC5/CSPα mutants causes lipofuscin accumulation. In Autophagy, 19, 204-223. doi:10.1080/15548627.2022.2065618. https://pubmed.ncbi.nlm.nih.gov/35506243/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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