C57BL/6JCya-Usp24em1flox/Cya
Common Name:
Usp24-flox
Product ID:
S-CKO-18651
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Usp24-flox
Strain ID
CKOCMP-329908-Usp24-B6J-VB
Gene Name
Product ID
S-CKO-18651
Gene Alias
2700066K03Rik; 2810030C21Rik; B130021E18
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Usp24em1flox/Cya mice (Catalog S-CKO-18651) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000094933
NCBI RefSeq
NM_183225
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Usp24, a deubiquitinase, plays diverse roles in various biological processes and diseases. It is involved in pathways such as NF-κB, autophagy, and regulation of protein stability, which are crucial for maintaining cell homeostasis and influencing disease progression [1-7, 9, 10]. Genetic models, like gene knockout in mice, are valuable for studying its functions.
In a high-fat diet and streptozotocin-induced mouse model of diabetic cardiomyopathy (DCM), knockdown of Usp24 reduced NF-κB levels, alleviating ferroptosis, as indicated by decreased fatty acid-CoA ligase 4 (FACL4), increased ferritin heavy chain 1 (FTH1) and solute carrier family 7 member 11 (SLC7A11), improved antioxidant capacity, and enhanced cell viability [1]. In a gefitinib-induced drug-resistant mouse model with doxycycline-induced EGFRL858R lung cancer, targeting Usp24 by USP24-i-101 inhibited drug resistance and activated autophagy, but this effect was abolished after autophagy inhibition [2]. In hepatocellular carcinoma, knockdown and overexpression studies showed that Usp24 regulates autophagy-dependent ferroptosis by reducing the K48-linked ubiquitination of Beclin1 [3]. In another study on hepatocellular carcinoma, Usp24 promoted tumorigenesis by deubiquitinating and stabilizing TRAF2, activating the AKT/NF-κB signaling pathway [4].
In conclusion, Usp24 is involved in multiple biological processes, with its dysregulation contributing to diseases like DCM, cancer drug resistance, and hepatocellular carcinoma. Gene knockout and other functional studies in mouse models have been instrumental in uncovering its role in these disease conditions, providing potential therapeutic targets for treatment.
References:
1. Wu, Shenglin, Zhou, Yueran, Liang, Jiaquan, Tan, Xuerui, Zhu, Jinxiu. 2023. Upregulation of NF-κB by USP24 aggravates ferroptosis in diabetic cardiomyopathy. In Free radical biology & medicine, 210, 352-366. doi:10.1016/j.freeradbiomed.2023.11.032. https://pubmed.ncbi.nlm.nih.gov/38056575/
2. Young, Ming-Jer, Wang, Shao-An, Chen, Yung-Ching, Lin, Shih-Min, Hung, Jan-Jong. 2024. USP24-i-101 targeting of USP24 activates autophagy to inhibit drug resistance acquired during cancer therapy. In Cell death and differentiation, 31, 574-591. doi:10.1038/s41418-024-01277-7. https://pubmed.ncbi.nlm.nih.gov/38491202/
3. Cao, Jiahui, Wu, Shitao, Zhao, Senfeng, Wang, Weijie, Sun, Yuling. 2024. USP24 promotes autophagy-dependent ferroptosis in hepatocellular carcinoma by reducing the K48-linked ubiquitination of Beclin1. In Communications biology, 7, 1279. doi:10.1038/s42003-024-06999-5. https://pubmed.ncbi.nlm.nih.gov/39379617/
4. Zhou, Nana, Guo, Chaoqin, Li, Xiangyu, Xu, Qiuran, Zheng, Xiaoliang. 2024. USP24 promotes hepatocellular carcinoma tumorigenesis through deubiquitinating and stabilizing TRAF2. In Biochemical pharmacology, 229, 116473. doi:10.1016/j.bcp.2024.116473. https://pubmed.ncbi.nlm.nih.gov/39127151/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen