C57BL/6JCya-Vps13cem1flox/Cya
Common Name:
Vps13c-flox
Product ID:
S-CKO-18654
Background:
C57BL/6JCya
Product Type
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Genotype
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Basic Information
Strain Name
Vps13c-flox
Strain ID
CKOCMP-320528-Vps13c-B6J-VB
Gene Name
Product ID
S-CKO-18654
Gene Alias
C230055H22Rik; chorein
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Vps13cem1flox/Cya mice (Catalog S-CKO-18654) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000077879
NCBI RefSeq
NM_177184
Target Region
Exon 3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
VPS13C, also known as PARK23, is a protein-coding gene. It encodes a lipid transfer protein that localizes to contact sites between the endoplasmic reticulum (ER) and late endosomes/lysosomes [2,3,6]. It plays a crucial role in regulating mitochondrial function through the endolysosomal pathway in neurons [1]. Mutations in VPS13C are associated with early-onset Parkinson's disease (PD) and dementia with Lewy bodies (DLB) [1].
Loss-of-function studies have provided key insights. Depleting VPS13C in HeLa cells causes lysosome accumulation with an altered lipid profile, activates the DNA-sensing cGAS-STING pathway due to elevated cytosolic mitochondrial DNA and defective activated STING degradation [2]. In human iPSC-derived dopaminergic neurons, loss of VPS13C disrupts lysosomal morphology, dynamics, motility, hydrolytic activity, and acidification, and decreases the phospho-Rab10-mediated lysosomal stress response [4]. Additionally, VPS13C deficiency in cervical cancer cells promotes cisplatin resistance by upregulating GSTP1 and inhibiting the JNK pathway [5].
In summary, VPS13C is essential for maintaining lysosomal and mitochondrial homeostasis. Studies on VPS13C, especially through loss-of-function models, have significantly enhanced our understanding of its role in PD and other diseases, such as cervical cancer's chemoresistance. These findings may help develop novel therapeutic strategies for related disorders.
References:
1. Monfrini, Edoardo, Spagnolo, Francesca, Canesi, Margherita, Pezzoli, Gianni, Di Fonzo, Alessio. 2021. VPS13C-associated Parkinson's disease: Two novel cases and review of the literature. In Parkinsonism & related disorders, 94, 37-39. doi:10.1016/j.parkreldis.2021.11.031. https://pubmed.ncbi.nlm.nih.gov/34875562/
2. Hancock-Cerutti, William, Wu, Zheng, Xu, Peng, Shadel, Gerald S, De Camilli, Pietro. 2022. ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling. In The Journal of cell biology, 221, . doi:10.1083/jcb.202106046. https://pubmed.ncbi.nlm.nih.gov/35657605/
3. Kumar, Nikit, Leonzino, Marianna, Hancock-Cerutti, William, Reinisch, Karin M, De Camilli, Pietro. 2018. VPS13A and VPS13C are lipid transport proteins differentially localized at ER contact sites. In The Journal of cell biology, 217, 3625-3639. doi:10.1083/jcb.201807019. https://pubmed.ncbi.nlm.nih.gov/30093493/
4. Schrӧder, Leonie F, Peng, Wesley, Gao, Ge, Schwake, Michael, Krainc, Dimitri. 2024. VPS13C regulates phospho-Rab10-mediated lysosomal function in human dopaminergic neurons. In The Journal of cell biology, 223, . doi:10.1083/jcb.202304042. https://pubmed.ncbi.nlm.nih.gov/38358348/
5. Tan, Xiangyu, Wang, Xueqian, Liao, Xueyao, Hu, Zheng, Tian, Xun. 2023. Downregulation of VPS13C promotes cisplatin resistance in cervical cancer by upregulating GSTP1. In iScience, 26, 107315. doi:10.1016/j.isci.2023.107315. https://pubmed.ncbi.nlm.nih.gov/37520723/
6. Cai, Shujun, Wu, Yumei, Guillén-Samander, Andrés, Liu, Jun, De Camilli, Pietro. 2022. In situ architecture of the lipid transport protein VPS13C at ER-lysosome membrane contacts. In Proceedings of the National Academy of Sciences of the United States of America, 119, e2203769119. doi:10.1073/pnas.2203769119. https://pubmed.ncbi.nlm.nih.gov/35858323/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen