C57BL/6JCya-Pfklem1flox/Cya
Common Name:
Pfkl-flox
Product ID:
S-CKO-18657
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Pfkl-flox
Strain ID
CKOCMP-18641-Pfkl-B6J-VB
Gene Name
Product ID
S-CKO-18657
Gene Alias
ATP-PFK; PFK-B; PFK-L
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pfklem1flox/Cya mice (Catalog S-CKO-18657) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020522
NCBI RefSeq
NM_008826
Target Region
Exon 8~11
Size of Effective Region
~4.0 kb
Detailed Document
Overview of Gene Research
PFKL, short for phosphofructokinase, liver type, is a key enzyme in glycolysis [2,3,4,5,6,7,8]. It plays a significant role in regulating glucose metabolism and is involved in multiple metabolic pathways. Glycolysis, in which PFKL participates, is crucial for energy production in cells, and abnormal regulation of PFKL can impact overall cellular metabolism and function. Genetic models, such as gene knockout mouse models, have been valuable in studying PFKL's functions.
In tumor cells, glucose deprivation phosphorylates PFKL, reducing its glycolytic activity and enabling it to interact with perilipin 2 (PLIN2). This leads to lipid droplet-mitochondria tethering, enhancing β-oxidation and tumor cell proliferation. Interfering with this cascade inhibits tumor cell growth [1]. In macrophages, phosphorylation of PFKL at Ser775 increases its catalytic activity, promoting glycolysis. In a genetic mouse model where PFKL Ser775 phosphorylation cannot occur, macrophage glycolysis is lower upon activation [4]. In cardiac hypertrophy in male mice, the KLF7/PFKL/ACADL axis modulates cardiac metabolic remodelling, with PFKL being a key target in the regulation of glycolysis and fatty acid oxidation fluxes [5].
In conclusion, PFKL is essential for the integrated regulation of glycolysis, lipid metabolism, and mitochondrial oxidation. Gene knockout mouse models have been instrumental in revealing its role in tumor growth, macrophage-mediated inflammation, and cardiac metabolic remodelling. Understanding PFKL's functions provides insights into disease mechanisms and potential therapeutic targets for related diseases such as cancer and cardiac hypertrophy.
References:
1. Meng, Ying, Guo, Dong, Lin, Liming, Xu, Daqian, Lu, Zhimin. 2024. Glycolytic enzyme PFKL governs lipolysis by promoting lipid droplet-mitochondria tethering to enhance β-oxidation and tumor cell proliferation. In Nature metabolism, 6, 1092-1107. doi:10.1038/s42255-024-01047-2. https://pubmed.ncbi.nlm.nih.gov/38773347/
2. Pan, Mingang, Luo, Muyu, Liu, Lele, Huang, Ailong, Xia, Jie. 2024. EGR1 suppresses HCC growth and aerobic glycolysis by transcriptionally downregulating PFKL. In Journal of experimental & clinical cancer research : CR, 43, 35. doi:10.1186/s13046-024-02957-5. https://pubmed.ncbi.nlm.nih.gov/38287371/
3. Zheng, Cancan, Yu, Xiaomei, Liang, Yiyao, He, Qingyu, Li, Bin. 2021. Targeting PFKL with penfluridol inhibits glycolysis and suppresses esophageal cancer tumorigenesis in an AMPK/FOXO3a/BIM-dependent manner. In Acta pharmaceutica Sinica. B, 12, 1271-1287. doi:10.1016/j.apsb.2021.09.007. https://pubmed.ncbi.nlm.nih.gov/35530161/
4. Wang, Meiyue, Flaswinkel, Heinrich, Joshi, Abhinav, Fröhlich, Thomas, Hornung, Veit. 2024. Phosphorylation of PFKL regulates metabolic reprogramming in macrophages following pattern recognition receptor activation. In Nature communications, 15, 6438. doi:10.1038/s41467-024-50104-7. https://pubmed.ncbi.nlm.nih.gov/39085210/
5. Wang, Cao, Qiao, Shupei, Zhao, Yufang, Chen, Yue, Tian, Weiming. 2023. The KLF7/PFKL/ACADL axis modulates cardiac metabolic remodelling during cardiac hypertrophy in male mice. In Nature communications, 14, 959. doi:10.1038/s41467-023-36712-9. https://pubmed.ncbi.nlm.nih.gov/36810848/
6. Chen, Shengmiao, Wu, Yiran, Gao, Yang, Zhao, Suwen, Fan, Gaofeng. 2023. Allosterically inhibited PFKL via prostaglandin E2 withholds glucose metabolism and ovarian cancer invasiveness. In Cell reports, 42, 113246. doi:10.1016/j.celrep.2023.113246. https://pubmed.ncbi.nlm.nih.gov/37831605/
7. Ma, Wenqi, Jia, Kangni, Cheng, Haomai, Zhang, Ruiyan, Yan, Xiaoxiang. 2024. Orphan Nuclear Receptor NR4A3 Promotes Vascular Calcification via Histone Lactylation. In Circulation research, 134, 1427-1447. doi:10.1161/CIRCRESAHA.123.323699. https://pubmed.ncbi.nlm.nih.gov/38629274/
8. Wang, Peng, Ye, Yixian, Chen, Zhaoyue, Hou, Guanghui, Liu, Zheng. 2024. PFKL promotes cell viability and glycolysis and inhibits cisplatin chemosensitivity of laryngeal squamous cell carcinoma. In Biochemical and biophysical research communications, 730, 150366. doi:10.1016/j.bbrc.2024.150366. https://pubmed.ncbi.nlm.nih.gov/38991254/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen