C57BL/6JCya-Vnn1em1flox/Cya
Common Name:
Vnn1-flox
Product ID:
S-CKO-18703
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Vnn1-flox
Strain ID
CKOCMP-22361-Vnn1-B6J-VC
Gene Name
Product ID
S-CKO-18703
Gene Alias
V-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Vnn1em1flox/Cya mice (Catalog S-CKO-18703) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041416
NCBI RefSeq
NM_011704
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Vnn1, also known as Vanin1, is an exogenous enzyme with pantetheinase activity. It degrades pantetheine to pantothenate (vitamin B5, a precursor of coenzyme A (CoA) biosynthesis) and cysteamine, and is involved in metabolism-related pathways such as glucolipid metabolism, cysteamine metabolism, and glutathione metabolism [2,4].
In Vnn1-related functional studies, VIVA mouse transgenic model (which specifically overexpresses Vnn1 on intestinal epithelial cells) was resistant to experimentally induced colitis, indicating Vnn1's role in enhancing mucosal protection in colitis. The pantetheinase activity of Vnn1 enhanced CoA regeneration and metabolic adaptation of colonocytes, and promoted microbiota-dependent production of short-chain fatty acids [1].
In AKI-CKD transition research, VNN1 knockout (KO) mice showed accelerated recovery of serum creatinine and blood urea nitrogen levels after ischemia/reperfusion (I/R) injury, inhibited renal fibrosis, and suppressed senescence of renal tubular cells after severe I/R injury, suggesting VNN1 promotes the AKI-CKD transition via inducing senescence of renal tubular cells [3].
In conclusion, Vnn1 plays a crucial role in various biological processes and disease conditions. Through gene knockout mouse models, its functions in mucosal protection during colitis and the AKI-CKD transition have been revealed. These findings provide insights into the underlying mechanisms of related diseases and potential therapeutic targets.
References：
1. Millet, Virginie, Gensollen, Thomas, Maltese, Michael, Naquet, Philippe, Galland, Franck. 2022. Harnessing the Vnn1 pantetheinase pathway boosts short chain fatty acids production and mucosal protection in colitis. In Gut, 72, 1115-1128. doi:10.1136/gutjnl-2021-325792. https://pubmed.ncbi.nlm.nih.gov/36175116/
2. Yu, Hao, Cui, Yuying, Guo, Fangyue, Dong, Deshi, Xiang, Hong. 2023. Vanin1 (VNN1) in chronic diseases: Future directions for targeted therapy. In European journal of pharmacology, 962, 176220. doi:10.1016/j.ejphar.2023.176220. https://pubmed.ncbi.nlm.nih.gov/38042463/
3. Chen, Jia, Lu, Hongxiang, Wang, Xiaoyue, He, Yani, Chen, Kehong. . VNN1 contributes to the acute kidney injury-chronic kidney disease transition by promoting cellular senescence via affecting RB1 expression. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36, e22472. doi:10.1096/fj.202200496RR. https://pubmed.ncbi.nlm.nih.gov/35959877/
4. Naquet, Philippe, Pitari, Giuseppina, Duprè, Sylvestro, Galland, Franck. . Role of the Vnn1 pantetheinase in tissue tolerance to stress. In Biochemical Society transactions, 42, 1094-100. doi:10.1042/BST20140092. https://pubmed.ncbi.nlm.nih.gov/25110008/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen