C57BL/6JCya-Sorl1em1flox/Cya
Common Name:
Sorl1-flox
Product ID:
S-CKO-18708
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Sorl1-flox
Strain ID
CKOCMP-20660-Sorl1-B6J-VB
Gene Name
Product ID
S-CKO-18708
Gene Alias
2900010L19Rik; LR11; SorLA; gp250; mSorLA
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sorl1em1flox/Cya mice (Catalog S-CKO-18708) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000060989
NCBI RefSeq
NM_011436
Target Region
Exon 5
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Sorl1, also known as LR11, is a type I transmembrane protein belonging to both the low-density lipoprotein receptor (LDLR) family and the vacuolar protein sorting 10 (VPS10) domain receptor family. It is genetically implicated in Alzheimer's disease (AD) and impacts amyloid precursor protein (APP) trafficking. Acting with the retromer trafficking complex, it regulates endosomal recycling of multiple transmembrane proteins related to AD pathophysiology, and is involved in lipid-related pathways through regulating APOE and CLU levels [1,2,3].
Sorl1-null induced pluripotent stem cells differentiated into different cell types show that Sorl1 loss leads to diverse pathway alterations, with the greatest effects in neurons and astrocytes. In neurons, it specifically reduces APOE and CLU and alters lipid profiles. Sorl1-knockout mouse models display learning and memory deficits, down-regulation of brain-derived neurotrophic factor in the hippocampus and cortex, and amyloid β-protein deposits. Hippocampal neuronal cell synapses from homozygous Sorl1-knockout mice are impaired, with reduced expression and colocalization of synaptic proteins [2,4].
In conclusion, Sorl1 is a key regulator in endosomal degradation and recycling pathways in neurons, and its loss-of-function significantly impacts AD-related biological processes. The use of gene-knockout models, especially Sorl1-KO mouse models, has revealed its crucial role in AD pathogenesis, providing insights for understanding the disease mechanism and potential therapeutic development for AD.
References:
1. Mishra, Swati, Knupp, Allison, Szabo, Marcell P, Andersen, Olav M, Young, Jessica E. 2022. The Alzheimer's gene SORL1 is a regulator of endosomal traffic and recycling in human neurons. In Cellular and molecular life sciences : CMLS, 79, 162. doi:10.1007/s00018-022-04182-9. https://pubmed.ncbi.nlm.nih.gov/35226190/
2. Lee, Hyo, Aylward, Aimee J, Pearse, Richard V, Young, Jessica E, Young-Pearse, Tracy L. 2023. Cell-type-specific regulation of APOE and CLU levels in human neurons by the Alzheimer's disease risk gene SORL1. In Cell reports, 42, 112994. doi:10.1016/j.celrep.2023.112994. https://pubmed.ncbi.nlm.nih.gov/37611586/
3. Yin, Rui-Hua, Yu, Jin-Tai, Tan, Lan. 2014. The Role of SORL1 in Alzheimer's Disease. In Molecular neurobiology, 51, 909-18. doi:10.1007/s12035-014-8742-5. https://pubmed.ncbi.nlm.nih.gov/24833601/
4. Zhao, Mingri, Chen, Xun, Liu, Jiangfeng, Liu, Mujun, Hou, Deren. 2023. Sorl1 knockout inhibits expression of brain-derived neurotrophic factor: involvement in the development of late-onset Alzheimer's disease. In Neural regeneration research, 19, 1602-1607. doi:10.4103/1673-5374.387975. https://pubmed.ncbi.nlm.nih.gov/38051905/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen