C57BL/6JCya-Zmym2em1flox/Cya
Common Name:
Zmym2-flox
Product ID:
S-CKO-18717
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Zmym2-flox
Strain ID
CKOCMP-76007-Zmym2-B6J-VB
Gene Name
Product ID
S-CKO-18717
Gene Alias
5830413P05Rik; FIM; MYM; RAMP; SCLL; Zfp198; Znf198
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zmym2em1flox/Cya mice (Catalog S-CKO-18717) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022511
NCBI RefSeq
NM_029498
Target Region
Exon 3
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Zmym2, also known as ZNF198, is a zinc finger transcriptional regulator and a component of transcriptional corepressor complexes. It is involved in multiple crucial biological processes. Zmym2 plays a role in the silencing of developmentally regulated endogenous retrovirus elements, and is associated with pathways related to DNA methylation, chromatin regulation, and cell identity maintenance [2,3,4].
Loss-of-function studies have been pivotal in understanding Zmym2's role. Heterozygous Zmym2 -deficient mice recapitulate features of congenital anomalies of the kidney and urinary tract (CAKUT) with high penetrance, indicating Zmym2's importance in renal development [1]. Zmym2 -/-mice show embryonic lethality by E10.5, with defects in DNA methylation and silencing of germline gene promoters and active LINE element subclasses [3]. In addition, zygotic depletion of Zmym2 compromises the totipotency -to -pluripotency transition during early development [2].
In conclusion, Zmym2 is essential for processes like DNA methylation patterning in early embryonic development, the totipotency -to -pluripotency transition, and renal development. Mouse models with Zmym2 knockout or knockdown have been instrumental in revealing its functions, especially in relation to CAKUT, providing insights into the pathogenesis of this disorder and potentially guiding new therapeutic strategies [1,2,3].
References:
1. Connaughton, Dervla M, Dai, Rufeng, Owen, Danielle J, Shril, Shirlee, Hildebrandt, Friedhelm. 2020. Mutations of the Transcriptional Corepressor ZMYM2 Cause Syndromic Urinary Tract Malformations. In American journal of human genetics, 107, 727-742. doi:10.1016/j.ajhg.2020.08.013. https://pubmed.ncbi.nlm.nih.gov/32891193/
2. Yang, Fan, Huang, Xin, Zang, Ruge, Zhou, Hongwei, Wang, Jianlong. . DUX-miR-344-ZMYM2-Mediated Activation of MERVL LTRs Induces a Totipotent 2C-like State. In Cell stem cell, 26, 234-250.e7. doi:10.1016/j.stem.2020.01.004. https://pubmed.ncbi.nlm.nih.gov/32032525/
3. Graham-Paquin, Adda-Lee, Saini, Deepak, Sirois, Jacinthe, Bouchard, Maxime, Pastor, William A. . ZMYM2 is essential for methylation of germline genes and active transposons in embryonic development. In Nucleic acids research, 51, 7314-7329. doi:10.1093/nar/gkad540. https://pubmed.ncbi.nlm.nih.gov/37395395/
4. Owen, Danielle J, Aguilar-Martinez, Elisa, Ji, Zongling, Li, Yaoyong, Sharrocks, Andrew D. 2023. ZMYM2 controls human transposable element transcription through distinct co-regulatory complexes. In eLife, 12, . doi:10.7554/eLife.86669. https://pubmed.ncbi.nlm.nih.gov/37934570/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen