C57BL/6JCya-Lipmem1flox/Cya
Common Name:
Lipm-flox
Product ID:
S-CKO-18738
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Lipm-flox
Strain ID
CKOCMP-78753-Lipm-B6J-VB
Gene Name
Product ID
S-CKO-18738
Gene Alias
4632427C23Rik; Lipl3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lipmem1flox/Cya mice (Catalog S-CKO-18738) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025685
NCBI RefSeq
NM_023903
Target Region
Exon 3~5
Size of Effective Region
~4.1 kb
Detailed Document
Overview of Gene Research
Lipm, also known as yqhM in Bacillus subtilis, is an octanoyltransferase. It is essential for the endogenous lipoylation of all lipoate-dependent proteins in B. subtilis, playing a crucial role in the lipoic acid biosynthesis pathway. LipM homologues are present in firmicutes and cyanobacteria. Understanding Lipm's function is important for clarifying the lipoic acid synthesis mechanism in these organisms [1,2].
In B. subtilis, a ΔlipM strain shows that LipM is absolutely required for the endogenous lipoylation of lipoate-dependent proteins. This indicates its vital role in lipoic acid biosynthesis. In contrast to Escherichia coli, B. subtilis requires LipM along with LipL for lipoic acid assembly. E. coli lipB can complement the growth of B. subtilis ΔlipL or ΔlipM strains in the absence of supplements, while an E. coli ΔlipB strain can be complemented with lipM but not lipL. These findings suggest that LipM and LipL catalyze sequential reactions in a novel lipoic acid biosynthesis pathway [1].
In conclusion, LipM is a key enzyme in the lipoic acid biosynthesis pathway in B. subtilis. Studies using gene-knockout-like models in B. subtilis, such as the ΔlipM strain, have revealed its essential role in lipoic acid synthesis, which is fundamental for understanding the metabolic processes related to lipoic acid in bacteria.
References:
1. Martin, Natalia, Christensen, Quin H, Mansilla, María C, Cronan, John E, de Mendoza, Diego. 2011. A novel two-gene requirement for the octanoyltransfer reaction of Bacillus subtilis lipoic acid biosynthesis. In Molecular microbiology, 80, 335-49. doi:10.1111/j.1365-2958.2011.07597.x. https://pubmed.ncbi.nlm.nih.gov/21338420/
2. Christensen, Quin H, Cronan, John E. 2010. Lipoic acid synthesis: a new family of octanoyltransferases generally annotated as lipoate protein ligases. In Biochemistry, 49, 10024-36. doi:10.1021/bi101215f. https://pubmed.ncbi.nlm.nih.gov/20882995/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen