C57BL/6JCya-Car7em1flox/Cya
Common Name:
Car7-flox
Product ID:
S-CKO-18746
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Car7-flox
Strain ID
CKOCMP-12354-Car7-B6J-VB
Gene Name
Product ID
S-CKO-18746
Gene Alias
Ca7
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Car7em1flox/Cya mice (Catalog S-CKO-18746) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000056051
NCBI RefSeq
NM_053070
Target Region
Exon 3~7
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
Car7, also known as carbonic anhydrase VII, is a gene encoding an enzyme belonging to the alpha-carbonic anhydrase family. These enzymes are involved in the catalysis of the reversible hydration of carbon dioxide, which is crucial for many physiological processes such as pH regulation, ion transport, and fluid balance [1,3]. In the body, Car7 is expressed in various tissues including the brain, kidneys, and digestive system, suggesting its involvement in multiple biological functions [1,2,4].
In a study using a Car7 knockout (KO) mouse model, it was found that mature hippocampal pyramidal neurons express Car7. P13-14 Car7 KO mice showed behavioral manifestations atypical of experimental febrile seizures (eFS) and a complete absence of electrographic seizures. This indicates that Car7 is a key molecule in age-dependent neuronal pH regulation, and its absence affects the generation of eFS. The study also found that Car7 mRNA is expressed in the human cerebral cortex before the age when febrile seizures occur in children [3].
In conclusion, Car7 plays an essential role in physiological processes, especially in neuronal pH regulation which is related to the occurrence of febrile seizures. The Car7 KO mouse model has significantly contributed to understanding the role of Car7 in this specific disease-related area, providing insights into the underlying mechanisms of febrile seizures at the genetic level.
References:
1. Lakkis, M M, O'Shea, K S, Tashian, R E. . Differential expression of the carbonic anhydrase genes for CA VII (Car7) and CA-RP VIII (Car8) in mouse brain. In The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45, 657-62. doi:. https://pubmed.ncbi.nlm.nih.gov/9154152/
2. Nagatani, Yusuke, Higashino, Toshihide, Kinoshita, Kosho, Higashino, Hideaki. 2021. Thromboxane A2 receptor antagonist (ONO-8809) attenuates renal disorders caused by salt overload in stroke-prone spontaneously hypertensive rats. In Clinical and experimental pharmacology & physiology, 48, 1391-1401. doi:10.1111/1440-1681.13543. https://pubmed.ncbi.nlm.nih.gov/34152603/
3. Ruusuvuori, Eva, Huebner, Antje K, Kirilkin, Ilya, Hübner, Christian A, Kaila, Kai. 2013. Neuronal carbonic anhydrase VII provides GABAergic excitatory drive to exacerbate febrile seizures. In The EMBO journal, 32, 2275-86. doi:10.1038/emboj.2013.160. https://pubmed.ncbi.nlm.nih.gov/23881097/
4. Pan, Pei-wen, Rodriguez, Alejandra, Parkkila, Seppo. 2007. A systematic quantification of carbonic anhydrase transcripts in the mouse digestive system. In BMC molecular biology, 8, 22. doi:. https://pubmed.ncbi.nlm.nih.gov/17362527/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen